Molecular evolutionary of hemagglutinin gene of influenza A (H1N1) pdm09 virus in Shandong Province from 2009 to 2024
10.3760/cma.j.cn112309-20240307-00078
- VernacularTitle:2009—2024年山东省甲型H1N1流感病毒血凝素基因进化分析
- Author:
Zhihong ZHAO
1
;
Yujie HE
;
Julong WU
;
Shaoxia SONG
;
Lin SUN
;
Zhong LI
;
Xianjun WANG
;
Zengqiang KOU
;
Hongling WEN
;
Ti LIU
Author Information
1. 山东大学齐鲁医学院公共卫生学院微生物检验学系，山东省"十四五"高等学校新发突发传染病防控与生物安全重点实验室，济南 250012
- Keywords:
Influenza A virus, H1N1 subtype;
Hemagglutinin gene;
Genetic evolution;
Amino acid variation
- From:
Chinese Journal of Microbiology and Immunology
2024;44(7):580-587
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To characterize and analyze the genetic variation of hemagglutinin (HA) of influenza A (H1N1) pdm09 subtype virus in Shandong Province, and explore the genetic variation patterns for providing reference for influenza monitoring, epidemic prevention and control, and vaccine strain selection.Methods:HA gene sequences of the recommended strains of influenza vaccine from 2009 to 2024 and the representative strains of each branch were downloaded from the GISAID Influenza Data Platform, and were phylogenetically analyzed and characterized in terms of amino acid site variation with the HA gene sequences of 298 influenza A (H1N1) virus strains isolated from Shandong Province. A phylogenetic tree was constructed using the maximum likelihood (ML) method of the IQ-TREE online tool, and the amino acid site variants were viewed using MegAlign software. The potential glycosylation sites of the HA gene were predicted using the NetNGlyc 1.0 online software.Results:The HA gene homology of the 298 influenza A (H1N1) viruses isolated in Shandong Province ranged from 91.2% to 100.0%. The evolutionary branches were gradually distantly related over time, but the direction of evolution was roughly the same as that in other provinces. Amino acid mutations in the HA occurred every year and most were found in the antigenic determinants.Conclusions:The HA genes of influenza viruses isolated in Shandong Province from 2009 to 2024 are still in the process of continuous evolution, and continuous monitoring of the epidemiological trends and the evolutionary directions of influenza viruses is essential for early warning of influenza virus pandemics.
