Protective effect of Bifidobacterium animalis BB69 on mice with inflammatory bowel disease
10.3760/cma.j.cn112309-20230530-00146
- VernacularTitle:动物双歧杆菌乳亚种BB69对炎症性肠病小鼠的保护作用
- Author:
Yingxiang HAN
1
;
Yuxin LIU
;
Weiqin ZHANG
Author Information
1. 内蒙古医科大学附属医院检验科，呼和浩特 010030
- Keywords:
Inflammatory bowel disease;
Bifidobacterium animalis BB69;
CD4 + T cells;
Cytokines;
Intestinal barrier
- From:
Chinese Journal of Microbiology and Immunology
2024;44(6):536-544
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct a mouse model of dextran sulfate sodium salt (DSS)-induced inflammatory bowel disease and investigate the protective effect of Bifidobacterium animalis BB69 using that model. Methods:Forty C57BL/6J mice were randomly divided into five groups: normal control group (NC group), model group (DSS group), BB69 treatment group (BB group), mesalamine treatment group (MS group), and BB69 combined with mesalamine group (BM). Except for the NC group, the other four groups were given drinking water containing 2.3% DSS for 7 d and the corresponding intervention. The condition of mice in each group was observed.Results:Compared with the mice in the DSS group, those in the BB and MS groups showed significantly reduced disease activity index ( P<0.05), improved colon shortening and edema ( P<0.05), alleviated intestinal mucosal damage and inflammatory cell infiltration, and increased expression of intestinal tight junction protein ( P<0.05). Besides, the differentiation of Treg cells and the expression of anti-inflammatory cytokines were enhanced ( P<0.05). Conclusions:Bifidobacterium animalis BB69 has a significant protective effect on mice with experimental inflammatory bowel disease.
