Identification and biological characterization of one Cupriavidus species isolated from human wound
10.3760/cma.j.cn112309-20231031-00115
- VernacularTitle:1株伤口分离贪铜菌的分类鉴定及生物学特征分析
- Author:
Shiyue KANG
1
;
Chenhui DENG
;
Xiaowei CHEN
;
Meilian LIN
;
Pinghua QU
;
Qiang ZHOU
Author Information
1. 广州中医药大学第二临床医学院，广州 510006
- Keywords:
Cupriavidus;
Species identification;
Whole genome sequencing
- From:
Chinese Journal of Microbiology and Immunology
2024;44(6):510-518
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the morphology and molecular biology and clarify the taxonomic status of one Cupriavidus species strain SZY C1 isolated from clinical wound specimens. Methods:Strain SZY C1 was subjected to physiological and biochemical identification, 16S rRNA gene sequencing, and whole genome sequencing. Its genomic features and virulence genes were analyzed using bioinformatics software.Results:Strain SZY C1 was a gram-negative, non-fermenting bacterium wihout flagella and the ability to form spores. After culturing on Columbia blood agar plates for 24 h, it formed grayish-white colonies that were round, raised, opaque, and had neatly defined margins. Based on 16S rRNA gene sequence analysis, strain SZY C1 belonged to the genus Cupriavidus with the highest 98.52% similarity to Cupriavidus metallicuns. The genome size of strain SZY C1 was determined to be 5 515 517 bp, with a G+ C content of 67.87%. Whole genome sequencing showed that strain SZY C1 had the closest phylogenetic relationship with Cupriavidus agavae, with an average nucleotide identity value of 84.76% and a digital DNA-DNA hybridization value of 29.1%, which were lower than the identification threshold for prokaryotic species. The strain SZY C1 carried multiple virulence genes, drug resistance genes, and heavy metal resistance genes. Conclusions:Based on phenotypic and genomic analyses, the strain SZY C1 is a potential new species of the Cupriavidus genus.
