Analysis of antibiotic resistance and infection of Bordetella pertussis in children with suspected pertussis and close family members
10.3760/cma.j.cn112309-20231225-00189
- VernacularTitle:百日咳疑似患儿及家属百日咳鲍特菌阳性情况及菌株耐药分析
- Author:
Lingbo WANG
1
;
Huaping WANG
;
Zhenghong QI
;
Pingping YAO
;
Shu TENG
;
Zhuoying WU
;
Beibei WU
;
Shiyong ZHAO
;
Zhangnyu YANG
Author Information
1. 浙江省疾病预防控制中心微生物检验科，浙江省传染病疫苗与预防控制研究重点实验室，杭州 310051
- Keywords:
Bordetella pertussis;
Close family members;
Antimicrobial resistance;
Resistance mechanism
- From:
Chinese Journal of Microbiology and Immunology
2024;44(6):473-479
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the positive rate and isolate Bordetella pertussis in children with suspected whooping cough and their close family members in Zhejiang Province, and further explore the susceptibility and resistant mechanism of Bordetella pertussis to antibiotics. Methods:A total of 273 nasopharynx swabs specimens from children with suspected whooping cough in Hangzhou Children′s Hospital from May 2022 to October 2022 were collected. The strains were isolated and cultured using charcoal select agar plate. Pertussis target genes were detected by RT-PCR. E-test method was used to detect the sensitivity of Bordetella pertussis strains to different antibiotics. The mechanism of resistance of Bordetella pertussis to macrolides was analyzed by whole genome sequencing. The phylogenetic analysis of isolated strains was based on core genome multilocus sequence typing(cgMLST). Results:Among 273 clinical samples of children with suspected pertussis and their close family members, 168 samples were positive by fluorescence quantitative PCR, accounting for 61.54%, and 30 pertussis strains were successfully isolated with a positive rate of 10.98%. In addition, among the 143 samples of close family members, 54.55% (78/143) samples were positive by RT-PCR and 9.79% (14/143) samples were positive by culture, suggesting that the close family member are important in family transmission of pertussis. Besides, most of the positive samples were from mothers. The results of E-test showed that 96.67%(29/30) strains showed high resistance to azithromycin with MIC value>256 mg/L, and the resistant mechanism of azithromycin was A2047G mutation in 23S rRNA. The phylogenetic analysis based on the cgMSLT showed that the isolated strains were clustered into two new different clades.Conclusions:The positive rate of Bordetella pertussis in close family members is at a high level and the mother may be the main source of infection, which is of great significance for monitoring and laboratory detection of suspected children′s family members. Bordetella pertussis shows high resistance to macrolides in Zhejiang Province, so monitoring of the antimicrobial resistance should be further strengthened to provide theoretical basis for clinical treatment and drug guidance.
