Genetic characterization of coxsackievirus A6 in Mianyang city in 2022
10.3760/cma.j.cn112309-20231101-00117
- VernacularTitle:2022年绵阳市柯萨奇病毒A6型基因特征分析
- Author:
Yue GONG
1
;
Kexin ZONG
;
Qian CHENG
;
Lulu WANG
;
Renhe ZHU
;
Qin LUO
;
Yanhai WANG
;
Guoyong MEI
;
Jun HAN
;
Qinqin SONG
Author Information
1. 内蒙古科技大学包头医学院公共卫生学院，包头 014000
- Keywords:
Hand, foot and mouth disease;
Pathogen spectrum;
Coxsackievirus group A type 6;
Genetic recombination;
Genetic evolution
- From:
Chinese Journal of Microbiology and Immunology
2024;44(5):414-422
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the etiology of hand, foot and mouth disease (HFMD) in Mianyang city in 2022, and to analyze the genetic characteristics of coxsackievirus A6.Methods:Pharyngeal swabs were collected from patients with HFMD in Mianyang city in 2022. Real-time fluorescence quantitative PCR was used to detect enteroviruses in the samples. Part of the VP1 gene in enterovirus-positive samples was amplified by nested PCR using enterovirus typing primers to further identify the viral types. The VP1 coding region of all CVA6-positive samples and the whole genome of some samples were amplified and sequenced by PCR. The endemic strain in Mianyang city was analyzed for phylogeny, gene homology, amino acid variation and genetic recombination.Results:A total of 151 pharyngeal swabs were collected, and 104 enterovirus-positive samples were detected by real-time fluorescence quantitative PCR, with an overall detection rate of 68.88% (104/151). The typing results showed that there were 77 cases of CVA6 infection, with a positive rate of 50.99% (77/151). The full-length VP1 genes of 77 CVA6 strains were amplified, sequenced, and successfully spliced, and phylogenetic analysis showed that all 77 strains were of the D3 genotype. There were multiple amino acid variant sites in the prevalent strains in Mianyang city compared with the reference strain. Twenty whole genome sequences were amplified, sequenced, and successfully spliced, and homology analysis showed that the nucleotide homology between the 20 positive sequences ranged from 97.0% to 99.9%. Phylogenetic tree and recombination analysis showed that no recombination occurred in the coding regions of the epidemic strains in this study.Conclusions:The predominant pathogen causing HFMD in Mianyang city in 2022 is CVA6 D3 subtype, which is consistent with the national epidemic in 2022.
