The effects of high-intensity exercise on cardiac remodeling and nitroso-redox imbalance in rats with diabetic cardiomyopathy
10.3760/cma.j.issn.0254-1424.2024.06.003
- VernacularTitle:高强度运动对糖尿病心肌病大鼠心脏重塑及亚硝基-氧化还原失衡的影响
- Author:
Jintao WU
1
;
Qiong WAN
;
Jing LIU
;
Weimin KANG
Author Information
1. 郑州工业应用技术学院，体育学院，新郑 451100
- Keywords:
High-intensity training;
Diabetic cardiomyopathy;
Cardiac remodeling;
Oxidative stress;
Nitroso-redox balance
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2024;46(6):500-505
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe any effect of high-intensity exercise on cardiac remodeling and nitroso-redox imbalance in rats modeling type 1 diabetes so as to provide a theoretical basis and targets for prescribing rehabilitation exercise for patients with diabetic cardiomyopathy.Methods:Forty-five 8-week-old healthy, male Sprague-Dawley rats were randomly divided into a normal control group (NC) of 10, a diabetic control group (DMC) and a diabetic exercise group (DME). Type 1 diabetes was induced in the DMC and DME groups by injecting streptozotocin. The NC and DMC group rats were then fed quietly in their cages, while the DME group rats performed high-intensity treadmill exercise 5 times a week for 4 weeks. Forty-eight hours after the last training, cardiac structure and function were detected by echocardiography, and fasting blood glucose was measured by the glucose oxidase method. Cardiomyocyte cross-sectional area (CSA) and interstitial collagen volume fraction (CVF) were observed. Myocardial tetrahydrobiopterin (BH4) content was determined chromatographically, and myocardial p47 phox and p67 phox protein, endothelial nitric oxide synthase (eNOS), and eNOS dimer and monomer protein levels were detected using western blotting. Results:Compared with the NC group, the average blood glucose, left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), myocardial CSA and CVF, and p47 phox and p67 phox protein expression had all increased significantly, but the eNOS dimer/monomer ratio and BH4 content had decreased significantly in the DMC group. In the DME group the average blood glucose, LVEDD, LVESD, myocardial CSA and CVF were significantly lower than in the DMC group, while there was no significant difference between the two groups in p47 phox or p67 phox protein expression, the eNOS dimer/monomer ratio or BH4 content. Conclusion:Short-term, high-intensity exercise can inhibit cardiac remodeling in rats with diabetic cardiomyopathy, but it does not relieve the nitro-redox imbalance.
