The effect of electroacupuncture pretreatment on the functioning of microvascular endothelial cells in the cerebrum after middle cerebral artery obstruction
10.3760/cma.j.issn.0254-1424.2024.06.002
- VernacularTitle:电针预处理对大脑中动脉栓塞小鼠脑微血管内皮细胞功能的影响
- Author:
Ying WANG
1
;
Jiang XU
;
Huihuang WANG
;
Hui WANG
;
Xiang ZHOU
;
Xuejing LI
Author Information
1. 徐州医科大学附属淮安医院，淮安 223022
- Keywords:
Electroacupuncture;
Cerebral ischemia and reperfusion;
YinYang-1 transcription factor;
p53 pathway
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2024;46(6):492-499
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe any effect of electroacupuncture pretreatment (EP) on the function of cerebral microvascular endothelial cells in mice with middle cerebral artery obstruction (MCAO), and to explore the role of miRNA-155 RNA and the p53 pathway in determining it.Methods:Forty-two mice were randomly divided into a sham operation group, a model group, an EP group, an miR-155 inhibitor (MI) group, an miR-155 agonist (MA) group, an miR-155 inhibitor negative control (MIN) group and an miR-155 agonist negative control (MAN) group. Each group had 6 rats. All except the sham-operated and model groups received EP, followed by injecting into the corresponding lateral ventricles inhibitor and agonist in groups MI and MA, and placebos in group MIN and MAN. All 7 groups were subjected to MCAO modeling 48 hours after the EP. Twenty-four and 72 hours after the modeling, neurological deficits were quantified using modified neurological severity scoring (mNSS). All of the mice were then sacrificed and any brain tissue damage was observed using hematoxylin-eosin (HE) staining. Terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) was used to observe any apoptosis of endothelial cells, and enzyme-linked immunosorbent assays (ELISA) were employed to detect the level of intercellular cell adhesion molecule-1 (ICAM-1) in the serum. Apoptotic endothelial cells in the cerebral microbleed and ICAM-1, Bcl-2 and Bax positive cells were detected using immunofluorescence. Quantitative real-time polymerase chain reactions (qRT-PCR) were carried to observe the expressions of miRNA-155 RNA and YY1 and p53 proteins in the ischemia/reperfusion (I/R) region.Results:Twenty-four and 72 hours after the MCAO the average mNSS score of the EP group was significantly lower than the model group′s average. HE staining showed that the cell morphology of the EP group was more regular. The number of apoptotic cells, the expression of ICAM-1, the relative expression of ICAM-1, the Bax/Bcl-2 ratio, as well as the relative expression of miRNA-155 RNA and YY1 and p53 proteins were all then significantly lower in the EP group than in the model group, on average.The expression of miRNA-155 RNA, p53 protein and ICAM-1, as well as the Bax to Bcl-2 ratio in the MI group was then significantly lower than in the MIN group, on average.Conclusion:Electroacupuncture pretreatment improves microvascular endothelial cell functioning in the cerebrum after ischemia and reperfusion (at least in mice) and may attenuate cerebral cell injury by down-regulating the miRNA-155-mediated YY1/p53 pathway.
