The effects of ointment and massage on inflammation, oxidative stress and angiogenesis after blunt skeletal muscle injury
10.3760/cma.j.issn.0254-1424.2024.05.003
- VernacularTitle:膏摩疗法对骨骼肌急性钝挫伤模型大鼠炎症及氧化应激和血管生成的影响
- Author:
Weixing ZHONG
1
;
Zujiang CHEN
;
Ning WANG
;
Junhua LI
;
Weijie PENG
;
Ruibin GU
;
Ziyu FENG
;
Yikai LI
Author Information
1. 南方医科大学中医药学院针灸推拿骨伤系，广州 510515
- Keywords:
Ointments;
Massage;
Skeletal muscles;
Muscle trauma;
Inflammation;
Oxidative stress;
Angiogenesis
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2024;46(5):401-406
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of ointment and massage on inflammation, oxidative stress and angiogenesis after skeletal muscle trauma, and to explore their mechanisms.Methods:Forty-two adult male Sprague-Dawley rats were randomly divided into a blank group ( n=6), an ointment and massage (O&M) group ( n=18) and a model group ( n=18). The blunt contusion model of gastrocnemius malformation was established in both the O&M and model groups using self-made percussion instruments. Two hours after successful modeling, the anti-inflammatory pain-relieving cream was applied to the injured area, and massaged evenly and gently for 5 minutes. That was repeated with an interval of 12 hours. No treatment was given to the model and blank groups. On the 1st, 3rd and 7th days after modeling, injured gastrocnemius muscles were resected after collecting abdominal blood. Hematoxylin-eosin (HE) staining and immunofluorescent (CD34) staining were applied, and serum superoxide dismutase (SOD) and malondialdehyde (MDA) contents were detected. Results:HE staining showed that at each time point the gastrocnemius muscle fibers of the model group were significantly more swollen and deformed, collapsed and dissolved than those of the blank group, with a large number of inflamed cells. The O&M group had better recovery, with more newly-generated muscle cells, less inflammatory infiltration and more normal cell shapes than the model group. Fluorescence was stronger in the O&M and model groups than in the blank group at each time point, with that of the O&M group significantly stronger than in the model group. The average SOD and MDA levels in the model and O&M groups were significantly higher than in the blank group, and on the 1st and 3rd days the O&M group′s average SOD level was significantly higher than the model group′s average, though by the 7th day there was no significant difference. The average MDA content of the O&M group was significantly lower than the model group′s average at each time point.Conclusion:Ointment and massage can effectively reduce the local inflammatory response and oxidative stress after a skeletal muscle injury. They can accelerate local angiogenesis, promoting the repair of damaged tissues.
