Metagenomic next-generation sequencing of cerebrospinal fluid assisting in the diagnosis of adult severe epidemic encephalitis B: a case report
10.3760/cma.j.cn113694-20231117-00324
- VernacularTitle:脑脊液宏基因组学二代测序协助诊断成人重型流行性乙型脑炎1例
- Author:
Jiali YANG
1
;
Jun JIN
;
Dongrong LI
;
Zhejun YU
;
Xinjing YANG
Author Information
1. 苏州大学附属第一医院重症医学科，苏州 215006
- Keywords:
Encephalitis, viral;
Encephalitis virus, Japanese;
Cerebrospinal fluid;
Metagenomic next-generation sequencing
- From:
Chinese Journal of Neurology
2024;57(8):890-893
- CountryChina
- Language:Chinese
-
Abstract:
The clinical manifestations of Japanese encephalitis virus infection are often acute encephalitis, and etiological diagnosis is a common diagnostic method. It is difficult to detect and isolate viruses clinically, and the types of pathogens detected are relatively limited. Metagenomics second-generation sequencing (mNGS) is a rapid and accurate molecular diagnostic method, which can improve the detection rate and types of pathogenic microorganisms. Pathogens uncommon in central nervous system infections can be detected. This paper reports a 27-year-old male patient with nausea and vomiting, high fever, disturbance of consciousness, and multiple organ failure as the main clinical manifestations. Japanese encephalitis virus was considered clinically, but etiological evidence was lacking. Japanese encephalitis virus was detected by cerebrospinal fluid mNGS, which greatly improved the accuracy of diagnosis of Japanese encephalitis virus infection.
