Novel analysis model for implant osseointegration using ectopic bone formation via the recombinant human bone morphogenetic protein-2/macroporous biphasic calcium phosphate block system in rats: a proof-of-concept study.
10.5051/jpis.2012.42.4.136
- Author:
Jung Chul PARK
1
;
Jong Bin LEE
;
Guy DACULSI
;
Sang Yeop OH
;
Kyoo Sung CHO
;
Gun Il IM
;
Byung Soo KIM
;
Chang Sung KIM
Author Information
1. Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, Korea. dentall@yuhs.ac
- Publication Type:Original Article
- Keywords:
Animals;
Dental implants;
Osseointegration
- MeSH:
Animals;
Bone Density;
Bone Morphogenetic Protein 2;
Calcium;
Dental Implants;
Humans;
Hydroxyapatites;
Male;
Nitrogen Mustard Compounds;
Osseointegration;
Osteogenesis;
Rats;
Rats, Sprague-Dawley;
Recombinant Proteins;
Titanium;
Transforming Growth Factor beta
- From:Journal of Periodontal & Implant Science
2012;42(4):136-143
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The osseointegration around titanium mini-implants installed in macroporous biphasic calcium phosphate (MBCP) blocks was evaluated after incubation with recombinant human bone morphogenetic protein-2 (rhBMP-2) in an ectopic subcutaneous rat model. METHODS: Mini-implants (phi1.8x12 mm) were installed in MBCP blocks (bMBCPs, 4x5x15 mm) loaded with rhBMP-2 at 0.1 mg/mL, and then implanted for 8 weeks into subcutaneous pockets of male Sprague-Dawley rats (n=10). A histomorphometric analysis was performed, and the bone-to-implant contact (BIC) and bone density were evaluated. RESULTS: Significant osteoinductive activity was induced in the rhBMP-2/bMBCP group. The percentage of BIC was 41.23+/-4.13% (mean+/-standard deviation), while bone density was 33.47+/-5.73%. In contrast, no bone formation was observed in the bMBCP only group. CONCLUSIONS: This model represents a more standardized tool for analyzing osseointegration and bone healing along the implant surface and in bMBCPs that excludes various healing factors derived from selected animals and defect models.
