Chemical constituents from the ethanol precipitated sediment of Radix Isatidis and their anti-inflammatory activities
10.3969/j.issn.1001-1528.2024.11.024
- VernacularTitle:板蓝根醇沉沉淀渣化学成分及其抗炎活性研究
- Author:
Kai-Hui LIU
1
;
Di WU
;
Shi-Min LI
;
Qiang XUE
;
Juan-Juan WU
;
Xue FENG
;
Xin HOU
;
Xin-Ying CHENG
Author Information
1. 河北晨光检测技术服务有限公司,河北邯郸 056107
- Keywords:
Radix Isatidis;
ethanol precipitated sediment;
chemical constituents;
anti-inflammatory activities;
synergistic manner;
comprehensive utilization
- From:
Chinese Traditional Patent Medicine
2024;46(11):3692-3697
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from the ethanol precipitated sediment of Radix Isatidis and their anti-inflammatory activities.METHODS The effects of ethanol precipitated sediment on IL-6 and TNF-α levels were detected by LPS-induced RAW264.7 cell inflammation model and enzyme-linked immunosorbent assay.The chemical constituents were analyzed by UPLC-QTOF-MS/MS and high performance molecular exclusion method.RESULTS When the concentration of Radix Isatidis ethanol sediment were 200 and 400 μg/mL,it could significantly inhibit the release of IL-6 and TNF-α.The ethanol sediment of Radix Isatidis was mainly composed of polysaccharides and proteins,including trace amounts of lipopeptides,indoles and amino acids,among which polysaccharides were mainly glucans.CONCLUSION The constituents from the ethanol sediment of Radix Isatidis are sugars,alkaloids(indoles),amino acids,and organic acids(fatty acids),and they may exert anti-inflammatory effects by synergistic manner.
