Effects of the compatibility of Caragana sinica Radix and Astragali Radix on a rat model of diabetic kidney disease via PINK1/MFN2/Parkin pathway
10.3969/j.issn.1001-1528.2024.11.012
- VernacularTitle:基于PINK1/MFN2/Parkin通路探讨金雀根和黄芪配伍对糖尿病肾病大鼠的影响
- Author:
Xian-Bing GUO
1
;
Yuan NIE
;
Cang-Cang XU
;
Yang ZHAO
;
Jian LIN
;
Ying-Jun DING
Author Information
1. 河北中医药大学,河北石家庄 050200
- Keywords:
Caragana sinica Radix;
Astragali Radix;
diabetic kidney diseases;
mitochondrial dynamics;
mitochondrial autophagy;
PINK1/MFN2/Parkin pathway
- From:
Chinese Traditional Patent Medicine
2024;46(11):3620-3628
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the impact of the combination use of Caragana sinica Radix and Astragali Radix on a rat model of diabetic kidney disease(DKD).METHODS The SD rats were randomly divided into the normal group,the model group,the Engelgin group,the Caragana sinica Radix group,the Astragali Radix group and the Caragana sinica Radix-Astragali Radix compatibility group,with 10 rats in each group.Following the successful establishment of a DKD model by unilateral amputate renal combined with intraperitoneal injection of streptozotocin(STZ),the corresponding gastric gavage of drugs were administered for 8 weeks.The rats had their 24 h urinary microalbumin(24 h U-mALB)detected at 0,4 and 8 weeks;their levels of Scr,BUN,CysC,MDA and SOD activity detected by ELISA;their renal ROS expression detected by fluorescence probe method;their renal pathological changes observed by HE,PAS,Masson and PASM-Masson staining;their renal expressions of NOX4,Drp1,MFN2 and P62 detected by immunohistochemistry;and their renal expressions of PINK1,MFN2,Parkin,LC3-Ⅱ/Ⅰ,P62 and p-Drp1 proteins detected by Western blot.RESULTS Compared with the model group,each treatment group displayed lower contents of 24 h U-mALB,BUN,Scr and CysC in the serum of rats(P＜0.01);reduced pathological structure damage of the renal tissue;decreased MDA level in serum and kidney(P＜0.01);increased SOD activity(P＜0.01);increased renal protein expressions of PINK1,MFN2,Parkin and LC3-Ⅱ/Ⅰ(P＜0.05,P＜0.01);and decreased protein expressions of p-Drp1 and P62(P＜0.01).And the Astragali Radix group and the Caragana sinica Radix-Astragali Radix compatibility group took the lead(P＜0.05,P＜0.01).CONCLUSION Upon the rat model of DKD,the compatibility of Caragana sinica Radix and Astragali Radix may alleviate their renal pathological damage and improve their renal function by activating the mitochondrial autophagy to improve mitochondrial dynamics and inhibit their oxidative stress via PINK1/MFN2/Parkin pathway.
