PI3K/Akt pathway-based investigation of total Astragalus saponins on sarcopenia in a rat model of type 2 diabetes mellitus
10.3969/j.issn.1001-1528.2024.11.011
- VernacularTitle:基于PI3K/Akt通路探讨黄芪总皂苷对2型糖尿病大鼠肌少症的影响
- Author:
Lei-Lei MA
1
,
2
;
Ji-An LI
;
Wen-Xuan XU
;
Jing-Ya WANG
;
Zhao-Yang TIAN
;
Jia-Yu LI
;
Ru-Jie HAN
;
Xiao-Jin LA
;
Chun-Yu TIAN
;
Hong CHANG
;
Zi-Yang DAI
;
Bi-Wei ZHANG
Author Information
1. 华北理工大学公共卫生学院,河北 唐山 063210
2. 华北理工大学中医学院,河北 唐山 063210
- Keywords:
total Astragalus saponins;
type 2 diabetes mellitus(T2DM);
sarcopenia;
PI3K/Akt pathway;
mTOR;
FoxO1
- From:
Chinese Traditional Patent Medicine
2024;46(11):3612-3619
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effects of total Astragalus saponins on the improvement of sarcopenia in a rat model of type 2 diabetes mellitus(T2DM).METHODS The rats were divided into the normal group for a normal feeding and the model group for the feeding of high-sugar and high-fat diet combined with intraperitoneal injection of STZ to establish a T2DM model.The successful model rats were randomly divided into the model group,the metformin group(0.2 g/kg)and the total Astragalus saponins group(80 mg/kg),and given corresponding doses of drugs by gavage.After 12 weeks administration,the rats had their FBG,postprandial blood glucose(PG2h)and wet weight of skeletal muscle measured;their serum levels of INS,C-peptide(C-P),IGF-1,TNF-α and IL-1β detected by ELISA;their morphological changes of skeletal muscle observed by HE staining;their protein expressions of PI3K,p-Akt,mTOR,S6K1,FoxO1 and Murf1 in skeletal muscle detected by Western blot;and their mRNA expressions of Pi3k,Akt and mtor in skeletal muscle detected by RT-qPCR method.RESULTS Compared with the model group,the total Astragalus saponins group displayed decreased levels of FBG,PG2h,OGTT-AUC,HOMA-IR,TNF-α and IL-1β(P＜0.01);increased levels of INS,C-P,IGF-1 and wet weight of skeletal muscle(P＜0.05,P＜0.01);improved skeletal muscle atrophy and increased protein expressions of PI3K,p-Akt,mTOR and S6K1 in skeletal muscle(P＜0.05,P＜0.01);decreased protein expressions of FoxO1 and Murf1(P＜0.05,P＜0.01);and increased mRNA expressions of Pi3k,Akt and mtor(P＜0.01).CONCLUSION The improvement effects of total Astragalus saponins on sarcopenia in T2DM rats may be associated with the regulation of PI3K/Akt/mTOR and PI3K/Akt/FoxO1 pathways.
