Analysis of constituents absorbed into blood and brain from Zhishe Tongluo Capsules
10.3969/j.issn.1001-1528.2024.11.007
- VernacularTitle:蛭蛇通络胶囊的入血、入脑成分研究
- Author:
Xiao-Yan ZHANG
1
;
Yang LIU
;
Xiao-Ting WANG
;
Hai-Feng WANG
;
Zhi-Biao DI
;
Jian-Fang SONG
;
Shi-Yu ZONG
;
Hong ZHANG
Author Information
1. 陕西省中医药研究院,陕西 西安 710003
- Keywords:
Zhishe Tongluo Capsules;
constituents absorbed into blood;
constituents absorbed into brain;
cerebral ischemia-reperfusion injury(MACO);
cerebral infarction;
UHPLC-Q Exactive Focus MS/MS
- From:
Chinese Traditional Patent Medicine
2024;46(11):3579-3584
- CountryChina
- Language:Chinese
-
Abstract:
AIM To analyze the constituents absorbed into blood and brain from Zhishe Tongluo Capsules.METHODS Sixteen rats were randomly assigned into four groups and given intragastric administration(3.1 g/kg),after which the cerebral ischemia-reperfusion injury(MACO)model was established,the blood and brain tissues were collected,and UHPLC-Q Exactive Focus MS/MS was adopted in the identification of prototype constituents.RESULTS Total 70 constituents were identified,20 of which were found in the blood,mainly including flavonoids,tanshinones and Ligusticum chuanxiong phthalides,and 7 of them could enter the brain through blood-brain barrier.Compared with the normal administration group,the MACO administration group demonstrated added constituents absorbed into blood containing 3-hydroxybenzoic acid,calycosin-7-glucoside,curcumenol,senkyunolide B,dihydrotanshinone I and cryptotanshinone;removed constituents absorbed into brain containing puerarin,elemicin,sedanolide,and added those containing salvianolic acid A,senkyunolide I,dihydrotanshinone I in the left brain tissues(infarcted side).CONCLUSION The constituents absorbed into blood and brain from Zhishe Tongluo Capsules,along with the enhanced absorptions of phthalides,quinones and phenols in MACO rats in vivo may be the active substances for treating cerebral infarction.
