Simultaneous determination of eight constituents in Lianhua Qingwen Capsules by LC-MS/MS

VernacularTitle:LC-MS/MS法同时测定连花清瘟胶囊中8种成分
Author: Piao-Ran QIN ^{1
,

2} ; Jia-Ye TIAN ; Su-Xia LI ; Fan GAO ; Wen-Hua YU ; Xing-Chao LIU ; Qiu-Hong GUO
Author Information

1. 河北中医药大学,河北石家庄 050200
2. 河北省高校中药开发与产业化应用技术研发中心,河北石家庄 050200
Keywords: Lianhua Qingwen Capsules; chemical constituents; LC-MS/MS
From: Chinese Traditional Patent Medicine 2024;46(11):3564-3568
CountryChina
Language:Chinese
Abstract: AIM To establish an LC-MS/MS method for the simultaneous content determination of forsythin,forsythoside A,chlorogenic acid,neochlorogenic acid,amygdalin,emodin,rhein and salidroside in Lianhua Qingwen Capsules.METHODS The analysis was performed on a 35℃thermostatic ACQUITY UPlC-HSS T3 column(100 mm×2.1 mm,1.8 μm),with the mobile phase comprising of 0.1%formic acid-acetonitrile flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in negative ion scanning with multiple reaction monitoring mode.RESULTS Eight constituents showed good linear relationships within their own ranges(r≥0.999 5),whose average recoveries were 99.20%-100.96%with the RSDs of 0.62%-1.23%.CONCLUSION This simple,sensitive and reliable method can be used for the quality control of Lianhua Qingwen capsules.