Simultaneous content determination of eleven constituents in Elephantopus scabre by TRSDMC
10.3969/j.issn.1001-1528.2024.11.003
- VernacularTitle:双标多测法同时测定地胆草中11种成分的含量
- Author:
Jia-Hui GUO
1
;
Le GAO
;
Ya-Qin ZHEN
;
Yu-Luo CAI
;
Jing-Nan HU
;
Wei TIAN
;
Li-Ying NIU
Author Information
1. 河北中医药大学,河北 石家庄 050091
- Keywords:
Elephantopus scabre L.;
chemical constituents;
content determination;
two reference substances for determination of multiple components(TRSDMC);
cluster analysis;
principal component analysis;
orthogonal partial least squares-discriminant analysis
- From:
Chinese Traditional Patent Medicine
2024;46(11):3557-3564
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a two reference substances for determination of multiple components(TRSDMC)method for the simultaneous content determination of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,luteolin-7-O-glucuronide,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C,deoxyelephantopin,isodeoxyelephantopin,isoscabertopin and scabertopin in Elephantopus scabre L..METHODS The analysis was performed on a 35℃thermostatic Waters Symmetry C18,Phenomenex C18,Agilent ZORBAX Eclipse Plus C18 columns(4.6 mm×250 mm,5.0 μm),with the mobile phase comprising of acetonitrile and 0.1%phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 220,326 nm.Chlorogenic acid was used as an internal standard to calculate the relative correction factors of neochlorogenic acid,cryptochlorogenic acid,luteolin-7-O-glucuronide,isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C,while isodeoxyelephantopin was used as an internal standard to calculate the relative correction factors of deoxyelephantopin,scabertopin and isoscabertopin,after which the content determination was made.Subsequently,cluster analysis,principal component analysis and orthogonal partial least squares-discriminant analysis were conducted.RESULTS Eleven constituents showed good linear relationships within their own ranges(r≥0.999 0),whose average recoveries were 95.3%-103.4%with the RSDs of 0.32%-3.45%.The result obtained by TRSDMC approximated those obtained by external standard method.Isochlorogenic acid A,isochlorogenic acid C,isochlorogenic acid B,chlorogenic acid,luteolin-7-O-glucuronide and cryptochlorogenic acid were taken as quality differential constituents.CONCLUSION This reliable and stable method can be used for the quality control of E.scabre.
