Simultaneou determination of twenty-eight constituents in Dayuan Drink by UPLC-MS/MS
10.3969/j.issn.1001-1528.2024.11.001
- VernacularTitle:UPLC-MS/MS法同时测定达原饮中28种成分
- Author:
Yu-Jie HOU
1
,
2
;
Xin-Jun ZHANG
;
Ming SU
;
Xin-Rui LI
;
Yue-Cheng LIU
;
Yu-Qing WANG
;
Dan-Dan SUN
;
Hui ZHANG
;
Kang-Ning XIAO
;
Long-Yun DUAN
;
Lei CAO
;
Zhen-Yu XUAN
;
Shan-Xin LIU
Author Information
1. 山东中医药大学药学院,山东 济南 250355
2. 山东省中医药研究院,山东 济南 250014
- Keywords:
Dayuan Drink;
chemical constituents;
UPLC-MS/MS
- From:
Chinese Traditional Patent Medicine
2024;46(11):3545-3552
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of gallic acid,protocatechuic acid,neomangiferin,catechin,caffeic acid,mangiferin,isomangiferin,albiflorin,paeoniflorin,vitexin,liquiritin,scutellarin,baicalin,liquiritigenin,timosaponin BⅡ,quercetin,wogonoside,benzoylpaeoniflorin,isoliquiritigenin,honokiol,magnolol,norarecaidine,arecaidine,arecoline,epicatechin,baicalein,glycyrrhizinate and wogonin in Dayuan Drink.METHODS The analysis was performed on a 35℃thermostatic Syncronis C18 column(100 mm×2.1 mm,1.7 μm),with the mobile phase comprising of 0.1%formic acid-acetonitrile flowing at 0.3 mL/min in a gradient elution manner,and electron spray inoization source was adopted in positive and negative ion scanning with select reaction monitoring mode.RESULTS Twenty-eight constituents showed good linear relationships within their own ranges(R2≥0.991 0),whose average recoveries were 95.60%-103.53%with the RSDs of 0.60%-5.45%.CONCLUSION This rapid,simple,selective,accurate and reliable method can be used for the quality control of Dayuan Drink.
