Effects of Didang Decoction-containing serum on high glucose-induced injury of rat glomerular endothelial cells
10.3969/j.issn.1001-1528.2024.09.013
- VernacularTitle:抵当汤含药血清对高糖诱导大鼠肾小球内皮细胞损伤的影响
- Author:
Bao-Lu LUO
1
;
Quan-Gen CHU
;
Jun CHU
;
Fei-Xiang LI
;
Jing CHEN
;
Yue-Qi WANG
Author Information
1. 安徽中医药大学中医学院,安徽 合肥 230012
- Keywords:
Didang Decoction;
rat glomerular endothelial cells(RGECs);
diabetic nephropathy;
Nrf2 signaling pathway;
oxidative stress
- From:
Chinese Traditional Patent Medicine
2024;46(9):2930-2935
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the protective effects of Didang Decoction-containing serum on rat glomerular endothelial cells(RGECs)following high glucose injury.METHODS Rats were given distilled water or Didang Decoction by gavage to prepare the blank serum or Didang Decoction-containing serum.CCK8 method was used to screen the glucose concentration for the modeling and serum concentration of the drug.The RGECs were divided into the blank group,the model group,Didang Decoction group(10%Didang Decoction medicated serum),Dapagliflozin group(normal serum+2 μmol/L Dapagliflozin)and Didang Decoction+Dapagliflozin group(10%Didang Decoction medicated serum+2 μmol/L Dapagliflozin).24 hrs after the drug treatment,the RGECs had their mRNA and protein expressions of Nrf2,HO-1 and NQO-1 detected by RT-qPCR method and Western blot method;their Nrf2 fluorescence expression detected by immunofluorescence method;and their SOD activity and MDA level detected by colorimetric method.RESULTS Compared with the blank group,the model group displayed decreased mRNA and protein expressions of Nrf2,HO-1 and NQO-1 as well as the activity of SOD(P＜0.01),and increased MDA level(P＜0.01).Compared with the model group,the groups intervened with the drugs showed increased mRNA and protein expressions of Nrf2,HO-1 and NQO-1 as well as the activity of SOD(P＜0.05,P＜0.01),and decreased MDA level(P＜0.01).CONCLUSION Didang Decoction-containing serum can protect RGECs from high glucose injury through activating the Nrf2 signaling pathway.
