Purification process for coumarins in Fraxini Cortex by macroporous resin
10.3969/j.issn.1001-1528.2024.09.007
- VernacularTitle:大孔树脂纯化秦皮香豆素工艺研究
- Author:
Dong-Xu ZHANG
1
;
Yong ZHANG
;
Si-Han XU-CHEN
;
Jia-Yi ZHOU
;
Le-Yang YU
;
Shen-Shu WANG
;
Tong ZHANG
;
Yue DING
Author Information
1. 上海中医药大学中药学院,上海 201203
- Keywords:
Fraxini Cortex;
coumarins;
purification process;
macroporous resin;
UPLC-QTOF-MS/MS
- From:
Chinese Traditional Patent Medicine
2024;46(9):2885-2891
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the purification process for esculin,fraxin,esculetin and fraxetin in Fraxini Cortex by macroporous resin.METHODS Static adsorption experiment was applied to screening resin model,single factor test was adopted in the optimization of purification process,UPLC-QTOF-MS/MS was used for identifying main components,after which heatmap was drawn.RESULTS The optimal resin model was ADS-5.The optimal purification process was determined to be 1.1 BV for loading amount,0.75 g/mL for loading concentration,2 BV pure water for washing impurity,and 4 BV 25%ethanol for eluting effective constituents,coumarins demonstrated the total transfer rate,purity and yield of 84.42%,53.28%and 4.79%,respectively.Total 37 constituents were identified,among which coumarins and phenylethanol glycosides were mainly concentrated in 25%ethanol eluent,organic acids,iridoids and flavonoids were mainly concentrated in 95%ethanol eluent.CONCLUSION This stable,feasible and accurate method can characterize the distribution patterns of coumarins in Fraxini Cortex in different eluents of macroporous resin,which provides guidance for further related pharmaceutical research.
