Identification of in vivo metabolites of Cynanchum auriculatum extract in functional dyspepsia rats by UHPLC Q-Exactive Plus Orbitrap HRMS
10.3969/j.issn.1001-1528.2024.09.006
- VernacularTitle:UHPLC Q-Exactive Plus Orbitrap HRMS法鉴定隔山消提取物在功能性消化不良大鼠体内代谢产物
- Author:
Zong-Qin WU
1
,
2
;
Jian GOU
;
Yong-Jun LI
;
Yuan LU
;
Qiao-Qiao RAN
;
Jia SUN
Author Information
1. 贵州医科大学省部共建药用植物功效与利用国家重点实验室/贵州省药物制剂重点实验室,贵州 贵阳 550004
2. 贵州医科大学药学院,贵州 贵阳 550004
- Keywords:
Cynanchum auriculatum Royle ex Wight extract;
metabolites;
functional dyspepsia;
UHPLC Q-Exactive Plus Orbitrap HRMS
- From:
Chinese Traditional Patent Medicine
2024;46(9):2876-2884
- CountryChina
- Language:Chinese
-
Abstract:
AIM To identify the in vivo metabolites of Cynanchum auriculatum Royle ex Wight extract in functional dyspepsia rats by UHPLC Q-Exactive Plus Orbitrap HRMS.METHODS The rat models for functional dyspepsia were established.The analysis was performed on a 40℃ thermostatic Hypersil GOLD C18 column(2.1 mm×100 mm,1.9 μm),with the mobile phase comprising of water(containing 0.1％formic acid)-acetonitrile(containing 0.1％formic acid)flowing at 0.3 mL/min in a gradient elution manner,and electrospray ionization source was adopted in positive and negative ion scanning.RESULTS Total 4 prototypes(baishouwubenzophenone,deacylmetaplexigenin,qingyangshengenin,syringic)and 110 metabolites were identified,12 of which were common metabolites in feces and urine,56 of which were unique metabolites in urine,42 of which were unique metabolites in feces.The metabolic pathway of prototypes contained phase Ⅰ metabolism(reduction,oxidization,etc.),phase Ⅱ metabolism(sulfonation,glucuronidation,etc.)and phase Ⅰ,Ⅱ composite reactions.CONCLUSION This effective and comprehensive method can lay the theoretical foundation for further discovery of potential active metabolites in C.auriculatum.
