Endophytic fungi from Scutellaria baicalensis and the enzyme inhibitory activities of their secondary metabolites
10.3969/j.issn.1001-1528.2024.08.025
- VernacularTitle:黄芩内生真菌及其次级代谢产物酶抑制活性研究
- Author:
De-Min LI
1
;
Xiao-Di MA
;
Kang-Xu WANG
;
Mei-Yuan LI
;
Man-Ping LUO
;
Ying-Ying MENG
;
Ai-Mei YANG
;
Bei WANG
;
Xin-Guo ZHANG
Author Information
1. 兰州理工大学生命科学与工程学院,甘肃省中藏药筛选评价及深加工重点实验室,甘肃 兰州 730050
- Keywords:
Scutellaria baicalensis Georgi;
endophytic fungi;
metabolites;
enzyme inhibitory activities;
tracking separation
- From:
Chinese Traditional Patent Medicine
2024;46(8):2644-2649
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study endophytic fungi from Scutellaria baicalensis Georgi.and the enzyme inhibitory activities of their secondary metabolites.METHODS Six different media were used to isolate and purify endophytic fungi from S.baicalensis by tissue homogenate method.The activities of secondary metabolites were evaluated by targeting different enzymes.The highly active strains were identified by molecular biology combined with morphology,and the highly active chemical components were tracked and separated by modern chromatographic separation technology.RESULTS Sixty-four endophytic fungal strains were isolated from S.baicalensis,and one hundred and twenty-eight secondary metabolites were obtained by fermentation.The samples with certain inhibitory activities against adenosine deaminase(ADA),β-lactamase and tyrosinase(TYR)accounted for 14.06%,3.91%and 18.75%,respectively.Strain HTS-23-2 showed high TYR inhibitory activity,and 99%homology with Aspergillus flavus by molecular identification.One compound was isolated from the fermentation samples and identified as kojic acid.CONCLUSION S.baicalensis harbors a rich diversity of endophytic fungi,which serve as a valuable resource for active substances.
