Chemical constituents from the large polar fraction of the roots of Lindera reflexa and their antitumor activities
10.3969/j.issn.1001-1528.2024.08.021
- VernacularTitle:山橿根大极性部位化学成分及其抗肿瘤活性研究
- Author:
Zhi-Hao TIAN
1
,
2
;
Xiao-Ya SUN
;
Shan-Shan ZHANG
;
Zhi-Ning GAO
;
Yan LI
;
Ruo-Xi CHEN
;
Sui-Qing CHEN
Author Information
1. 河南中医药大学,河南 郑州 450046
2. 河南省中药资源与中药化学重点实验室,河南 郑州 450046
- Keywords:
Lindera reflexa Hemsl;
roots;
chemical constituents;
isolation and identification;
antitumor activities
- From:
Chinese Traditional Patent Medicine
2024;46(8):2617-2623
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from the large polar fraction of the roots of Lindera reflexa Hemsl.and their antitumor activities.METHODS The large polar fraction from the roots of L.reflexa was isolated and purified by silica gel column,Sephadex LH-20 gel column,semi-preparative HPLC and ODS medium pressure column,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antitumor activities were determined by MTT method.RESULTS Thirteen compounds were isolated and identified as 2,6-dimethoxy-4-hydroxyphenyl-1-O-β-D-glucopyranoside(1),3-hydroxy-4,5-dimethoxyphenol-β-D-glucopyranoside(2),syringin(3),1-O-3,4-dimethoxy-5-hydroxyphenyl-(6-O-3,5-dimethoxygalloyl)-β-D-glucopyranoside(4),p-cymen-7-yl β-D-glucopyranoside(5),pisumionoside(6),staphylionoside D(7),dendranthemoside B(8),lynoiside(9),nudiposide(10),icariside B1(11),(2S)-pinocembrin-7-O-(6-O-α-L-rhamnopyranosyl-β-D-glucopyranoside)(12),(+)-N-(methoxycarbonyl)-N-norboldine(13).Compounds 3 and 13 showed obvious cytotoxicity against human lung cancer cells(A549)and human gastric cancer cells(MGC80-3).CONCLUSION Compounds 1-13 are isolated from the roots of L.reflexa for the first time.Compounds 3 and 13 have good anti-tumor activities.
