Extraction process optimization and content determination of eight nucleosides from Pheretima guillelmi
10.3969/j.issn.1001-1528.2024.08.006
- VernacularTitle:威廉环毛蚓中8种核苷提取工艺优化及含量测定
- Author:
Quan-Lin YU
1
;
Xue-Chun WU
;
Yi QIU
;
Jia-Jia SONG
;
Qiao-Ping JIANG
;
Chang-Sheng SUN
;
Jing-Nan WU
;
Cheng-Ke CAI
;
Hong-Fei WANG
Author Information
1. 北京中医药大学中药学院,北京 102400
- Keywords:
Pheretima guillelmi(Michaelsen);
nucleosides;
extraction process;
content determination;
orthogonal test;
HPLC
- From:
Chinese Traditional Patent Medicine
2024;46(8):2526-2530
- CountryChina
- Language:Chinese
-
Abstract:
AIM To optimize the extraction process for uracil,hypoxanthine,xanthine,uridine,thymine,inosine,guanosine and 2'-deoxyguanosine from Pheretima guillelmi(Michaelsen),and to determine their contents.METHODS With solid-liquid ratio,ultrasonic time and ultrasonic temperature as influencing factors,contents of hypoxanthine and total nucleosides as evaluation indices,the extraction process was optimized by orthogonal test.HPLC was adopted in the content determination of varioud nucleosides,the analysis was performed on a 30℃thermostatic Agilent C18 column(4.6 mm×250 mm,5 μm),with the mobile phase comprising of methanol-water flowing at 1 mL/min in a gradient elution manner,and the detection wavelength was set at 260 nm.RESULTS The optimal conditions were determined to be 1∶250 for solid-liquid ratio,60 min for ultrasonic time,and 60℃for ultrasonic temperature.Eight nucleosides showed good linear relationships within their own ranges(R2＞0.999 0),whose average recoveries were 99.11%-103.27%with the RSDs of 0.85%-2.89%.CONCLUSION This stable and reliable method can be used for the extraction and content determination of nucleosides from P.guillelmi.
