Analyses of chemical constituents and constituents absorbed into blood from Qili Qiangxin Capsules by UPLC-QTOF/MS
10.3969/j.issn.1001-1528.2024.08.005
- VernacularTitle:UPLC-QTOF/MS法分析芪苈强心胶囊化学成分及入血成分
- Author:
Kuang-Yi LIU
1
;
Ling-Yun ZHANG
;
Yan-Ting XIONG
;
Mi PENG
;
Hui CHEN
Author Information
1. 南昌市第一医院,江西 南昌 330000
- Keywords:
Qili Qiangxin Capsules;
chemical constituents;
constituents absorbed into blood;
UPLC-QTOF/MS
- From:
Chinese Traditional Patent Medicine
2024;46(8):2517-2525
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a UPLC-QTOF/MS method for the analyses of chemical constituents and constituents absorbed into blood from Qili Qiangxin Capsules by UPLC-QTOF/MS.METHODS The rats with heart failure after myocardial infarction were given intragastric administration of 0.5%CMC-Na suspension of Qili Qiangxin Capsules(crude drug dosage was 0.9 g/mL)for 4 weeks,after which blood collection was made.The analysis was performed on a 40℃thermostatic Acquity UPLC BEH C18 Column(100 mm×2.1 mm,1.7 μm),with the mobile phase comprising of 0.1%formic acid-acetonitrile(positive ion manner)or water-acetonitrile(negative ion manner)flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning.RESULTS Total 151 constituents were identified,containing 38 flavonoids,36 terpenoids,23 alkaloids,11 saponins,11 phenylpropanol,10 phenolic acids,4 organic acids,4 cardiac glycosides and 14 others,along with 35 constituents absorbed into blood.CONCLUSION This accurate and stable method can provide data support for the rational clinical application of Qili Qiangxin Capsules,and lay foundation for the research on related effector substances of other Chinese traditional patent medicines.
