Simultaneous content determination of seven constituents in Anshen Capsules by HPLC
10.3969/j.issn.1001-1528.2024.08.003
- VernacularTitle:HPLC法同时测定安神胶囊中7种成分的含量
- Author:
Shuai CUI
1
,
2
;
Jun-Feng CUI
;
Xiu-Kun LIANG
;
Zhi-Yong WANG
;
Qing XIA
Author Information
1. 滨州市检验检测中心,山东 滨州 256600
2. 滨州市化学药物研发与质量控制重点实验室(筹),山东 滨州 256600
- Keywords:
Anshen Capsules;
chemical constituents;
content determination;
HPLC;
principal component analysis;
partial least squares discriminant analysis
- From:
Chinese Traditional Patent Medicine
2024;46(8):2506-2510
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of spinosin,ferulic acid,rosmarinic acid,salvianolic acid B,schisandrin A,schizandrin B and schisandrol A in Anshen Capsules.METHODS The analysis was performed on a 30℃thermostatic Inertsustain C18 column(5 μm,4.6 mm×250 mm),with the mobile phase comprising of methanol-acetonitrile-0.1%phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 203,270 nm.Subsequently,principal component analysis and partial least squares discriminant analysis were made.RESULTS Seven constituents showed good linear relationships within their own ranges(r＞0.999 0),whose average recoveries were 96.12%-102.70%with the RSDs of 0.31%-1.83%.Ten batches of samples were divided into two categories according to manufacturers,and peaks 5(schisandrol A),4(salvianolic acid B)were quality difference markers.CONCLUSION This simple,sensitive,specific and reproducible method can be used for the quality control of Anshen Capsules.
