Analyses of chemical constituents and constituents absorbed into blood from Runing Granules by UPLC-QTOF/MS

VernacularTitle:UPLC-QTOF/MS法分析乳宁颗粒化学成分及入血成分
Author: Mi PENG ¹ ; Ling-Yun ZHANG ; Kuang-Yi LIU
Author Information

1. 南昌市人民医院 (南昌市第三医院),江西南昌 330000
Keywords: Runing Granules; chemical constituents; constituents absorbed into blood; UPLC-QTOF/MS
From: Chinese Traditional Patent Medicine 2024;46(7):2163-2172
CountryChina
Language:Chinese
Abstract: AIM To establish a UPLC-QTOF/MS method for the analyses of chemical constituents and constituents absorbed into blood from Runing Granules.METHODS Two rats were given intragastric administration of the aqueous solution of granules,after which blood collection was made at 0,0.5,1,2,4 h.The analysis was performed on a 40℃ thermostatic Acquity UPLC BEH C18 column(100 mm×2.1 mm,1.7 μm),with the mobile phases comprising of 0.1% formic aci D-acetonitrile(positive ion)or water-acetonitrile(negative ion)flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning.RESULTS Total 166 chemical constituents were identified,containing 44 flavonoids,17 terpenes,23 alkaloids,11 saponins,29 phenolic acids,13 quinones,10 organic acids,and 19 others,along with 11 constituents absorbed into blood.CONCLUSION This accurate and stable method can provide a data support for the clinical application of Runing Granules,and lay foundations for the further researches on their quality control and material basis.