Simultaneous content determination of twelve constituents in Naomaitai Capsules by HPLC
10.3969/j.issn.1001-1528.2024.07.004
- VernacularTitle:HPLC法同时测定脑脉泰胶囊中12种成分的含量
- Author:
Jun-Qing GAO
1
;
Xian-Song CHENG
;
Shu-Qi YU
;
Ni ZHANG
Author Information
1. 陕西省人民医院神经内一科,陕西 西安 710068
- Keywords:
Naomaitai Capsules;
chemical constituents;
content determination;
HPLC;
cluster analysis;
principal component analysis
- From:
Chinese Traditional Patent Medicine
2024;46(7):2147-2152
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of 3′-hydroxy-puerarin,hydroxysafflor yellow A,puerarin,puerarin apigenin,3′-methoxypuerarin,daidzein,stilbene glycoside,luteolin side,genistein side,luteolin B and rosmarinic acid in Naomaitai Capsules.METHODS The analysis was performed on a 30℃ thermostatic Kromasil C18 column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-water(containing 1.0 g/L phosphoric acid)flowing at 0.8 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.Subsequently,principal component analysis and cluster analysis were made.RESULTS Twelve components showed good linear relationships within their own ranges(r＞0.999 0),whose average recoveries were 97.00%-99.00% with the RSDs of 0.86%-1.82% .Ten batches of samples were clustered into four categories,the accumulative variance contribution rate of four principal components reached 86.262% .CONCLUSION This simple,stable,reliable and accurate method can be used for the quality control of Naomaitai Capsules.
