Simultaneous content determination of eighteen nucleosides and free amino acids in Colla corii asini by UPLC-MS/MS
10.3969/j.issn.1001-1528.2024.07.003
- VernacularTitle:UPLC-MS/MS法同时测定阿胶中18种核苷、游离氨基酸的含量
- Author:
Shuai YANG
1
,
2
;
Lin ZHENG
;
Ming-Yan CHI
;
Zi-Peng GONG
;
Yue-Ting LI
;
Mao-Chen WEI
;
Yong HUANG
Author Information
1. 贵州医科大学,贵州省药物制剂重点实验室/药用植物功效与利用国家重点实验室,贵州 贵阳 550004
2. 贵州医科大学药学院,贵州 贵阳 550004
- Keywords:
Asini Corii Colla;
nucleosides;
free amino acids;
content determination;
UPLC-MS/MS;
chemical pattern recognition
- From:
Chinese Traditional Patent Medicine
2024;46(7):2140-2146
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of Asp,Guad,Adeno,Arg,Ade,Cyti,Phe,Leu,Ile,Glu,Ser,Gln,Gly,Ala,Hyp,Thr,Pro and Lys in Asini Corii Colla.METHODS The analysis was performed on a 45℃ thermostatic Waters BEH C18column(2.1 mm×50 mm,1.7 μm),with the mobile phase comprising of acetonitrile(containing 0.1% formic acid)-water flowing at 0.35 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive ion scanning with multiple reaction monitoring mode.Subsequently,chemical pattern recognition was performed by hierarchical clustering analysis,principal component analysis and orthogonal partial least squares-discriminant analysis.RESULTS Eighteen nucleosides and free amino acids showed good linear relationships within their own ranges(r≥0.999 0),whose average recoveries were 98.0%-104.9% with the RSDs of 1.6%-4.9% .Seventeen batches of samples were clustered into two categories,two principal components demonstrated the accumulative variance contribution rate of 60.75%,Leu,Phe,Ade and Guad were potential index constituents.CONCLUSION This stable and reliable method can be used for the quality control of Asini Corii Colla.
