Quality contol of Bupleurum chinense
10.3969/j.issn.1001-1528.2024.07.001
- VernacularTitle:北柴胡质量控制研究
- Author:
Jing-Bo YU
1
;
Yue HAN
;
Qi-Di AI
;
Yang SUN
;
Zi-Yang ZHOU
;
Sai-Hu LIU
;
Zi-Hao ZHANG
;
Hui SHU
;
Yu-Hong WANG
Author Information
1. 湖南中医药大学科技创新中心/中药粉体与创新药物省部共建国家重点实验室培育基地,湖南 长沙 410208
- Keywords:
Bupleurum chinense DC.;
quality control;
HPLC fingerprints;
content determination;
principal component analysis
- From:
Chinese Traditional Patent Medicine
2024;46(7):2129-2133
- CountryChina
- Language:Chinese
-
Abstract:
AIM To control the quality of Bupleurum chinense DC.METHODS The analysis was performed on a 35℃ thermostatic Venusil XBP C18 column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-water flowing at 1.0 mL/min,and the detection wavelength was set at 210 nm.The HPLC fingerprints were established,after which the contents of saikosaponin A,saikosaponin B2,saikosaponin C,saikosaponin D,saikosaponin E,saikosaponin F and 6″-O-acetylsaikosaponin A were determined,and principal component analysis was made.RESULTS There were thirteen common peaks in the fingerprints for twelve batches of medicinal materials with the similarities of 0.970-0.995.Seven constituents showed good linear relationships within their own ranges(R2≥0.999 8),whose average recoveries were 90.75%-100.91% with the RSDs of 1.6%-4.0% .Various constituents demonstrated similar contents in medicinal materials originated in Inner Mongolia and Shanxi.CONCLUSION This precise,accurate and stable method can be used for the quality evaluation of B.chinense.
