Chemical constituents from the methanol fraction of Premna fulva leaves and their anti-inflammatory activities
10.3969/j.issn.1001-1528.2024.06.020
- VernacularTitle:战骨叶甲醇部位化学成分及其抗炎活性研究
- Author:
Fei-Fan HE
1
,
2
;
Ning-Tao ZHAO
;
Xiao-Jie YAN
;
Feng-Lai LU
;
Xiao-Hua JIANG
;
He-Ping LI
;
Dian-Peng LI
;
Yue-Yuan CHEN
Author Information
1. 广西壮族自治区中国科学院广西植物研究所,广西植物功能物质与资源持续利用重点实验室,广西桂林 541006
2. 桂林理工大学,广西桂林 541006
- Keywords:
leaves of Premna fulva Craib;
methanol fraction;
chemical constituents;
isolation and identification;
anti-inflammatory activities
- From:
Chinese Traditional Patent Medicine
2024;46(6):1894-1899
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from the methanol fraction of Premna fulva Craib leaves and their anti-inflammatory activities.METHODS The methanol fraction from P.fluva were isolated and purified by TLC,column chromatography,and HSCCC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their anti-inflammatory activities in vitro were evaluated by RAW264.7 model.RESULTS Twelve compounds were isolated and identified as vitexin(1),balanophonin(2),inotodisaccharide(3),4-allyl-2,6-dimethoxyphenol-β-D-glucopyranoside(4),dehydrovomifoliol(5),loliolide(6),(E)-4-((1S,3R,4R)-1-hydroxy-4,5,5-trimethyl-7-oxabicyclo[4.1.0]heptan-1-yl)but-1-en-3-o-ne(7),(E)-4-hydroxyphenylprop-7-ene 4-O-β-D-glucopyranoside(8),4-O-β-D-glucopyranosylbenzoic acid(9),vicenin(10),oleanicacid(11),sesamin(12),respectively.Compounds 1,2,5,7,10,and 12 showed good inhibitory activities against NO,and the IC50 values were(26.42±2.5)、(21.24±2.2)、(25.88±1.9)、(29.72±2.1)、(8.90±1.1)、(9.73±0.7)μmol/L,respectively.CONCLUSION Compounds 2,4-8 are isolated from Premna genus plants for the first time.Compounds 1,2,5,7,10,12 have anti-inflammatory activities.
