Chemical constituents from Ligularia duciformis and their anti-inflammatory activities
10.3969/j.issn.1001-1528.2024.06.018
- VernacularTitle:大黄橐吾化学成分及其抗炎活性研究
- Author:
Ri-Cuo LUO
1
;
Xin-Yu ZHANG
;
Guo-Yue ZHONG
;
Hong-Ling WANG
Author Information
1. 江西中医药大学中药资源与民族药研究中心,江西南昌 330004
- Keywords:
Ligularia duciformis(C.Winkl.)Hand.-Mazz.;
chemical constituents;
isolation and identification;
anti-inflammatory activities
- From:
Chinese Traditional Patent Medicine
2024;46(6):1882-1888
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from Ligularia duciformis(C.Winkl.)Hand.-Mazz.and their anti-inflammatory activities.METHODS The ethanol extract from L.duciformis was isolated and purified by HPLC,gel column and silica gel chromatography,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The anti-inflammatory activities of the compounds in vitro was determined by CCK-8 and Griess methods.RESULTS Eighteen compounds were isolated and identified as 14-acetoxy-7β-senecioyloxy-1-(2-methylbutyryloxy)-notonipetranone(1),rel-(1R,3αS,5R,6S,7R,7αS)-1-[(1S)-1-(acetyloxy)ethyl]octahydro-6-[(2-methylbutanoyl)oxy]-4-methylidene-2-oxo-7-(propan-2-yl)-1H-inden-5-yl(2E)-3-methylpent-2-enoate(2),tussilagone(3),petasipaline B(4),8α-hydroxy-4(15),11-eudesmadiene(5),lupenone(6),lupeol(7),(3[3)-lup-20(29)-en-3-yl stearate(8),pinoresinol monoglucoside(9),isoeucommin A(10),isoline(11),uridine(12),tetratriacontanol(13),palmitic acid(14),tridec-1-ene(15),cis-octadec-9-enoic acid(16),methyl-α-D-fructofuranoside(17),β-sitosterol(18).Compounds 1-6,9-11 and 14 showed inhibitory activities against LPS-induced NO production in RAW264.7 cells.CONCLUSION Compounds 3-6,8-10,12-17 are first isolated from this plant.Compounds 1-6,9-11 and 14 have anti-inflammatory activities.
