Quality evaluation of Yanyangke Mixture
10.3969/j.issn.1001-1528.2024.06.002
- VernacularTitle:咽痒咳合剂质量评价
- Author:
Xiao-Lian LIANG
1
,
2
;
Xiong-Bin GUI
;
Yong CHEN
;
Zheng-Teng YANG
;
Jia-Bao MA
;
Feng-Xian ZHAO
;
Hai-Mei SONG
;
Jia-Ru FENG
Author Information
1. 广西中医药大学,广西南宁 530200
2. 梧州市中医医院,广西梧州 543000
- Keywords:
Yanyangke Mixture;
quality evaluation;
HPLC fingerprints;
cluster analysis;
principal component analysis;
partial least squares discriminant analysis;
content determination
- From:
Chinese Traditional Patent Medicine
2024;46(6):1781-1787
- CountryChina
- Language:Chinese
-
Abstract:
AIM To evaluate the quality of Yanyangke Mixture.METHODS The HPLC fingerprints were established,after which cluster analysis,principal component analysis and partial least squares discriminant analysis were performed.The contents of liquiritin,rosmarinic acid,sheganoside,irisgenin,honokiol,monoammonium glycyrrhizinate,irisflorentin,isoliquiritin and magnolol were determined,the analysis was performed on a 35 ℃ thermostatic Agilent ZORBAX SB-C18 column(5 μm,250 mmx4.6 mm),with the mobile phase comprising of 0.1％phosphoric acid-acetonitrile flowing at 1 mL/min in a gradient elution manner,and multi-wavelength detection was adopted.RESULTS There were ten common peaks in the fingerprints for twelve batches of samples with the similarities of more than 0.9.Various batches of samples were clustered into three types,three principal components displayed the acumulative variance contribution rate of 87.448％,peaks 5、14(honokiol),3(liquiritin),11(monoammonium glycyrrhizinate)and 15(asarinin)were quality markers.Nine constituents showed good linear relationships within their own ranges(r＞0.999 0),whose average recoveries were 98.5％-103.6％with the RSDs of 0.92％-1.7％.CONCLUSION This stable and reliable method can provide a basis for the quality control of Yanyangke Mixture.
