Chemical constituents from Cleidion brevipetiolatum and their anti-inflammatory activities
10.3969/j.issn.1001-1528.2024.05.019
- VernacularTitle:棒柄花化学成分及其抗炎活性研究
- Author:
Wei WEI
1
;
Jin-Ni TAN
;
Min-Jing LI
;
Chun-Hua LAI
;
Qin-Gang TAN
Author Information
1. 桂林医学院,广西 桂林 541199
- Keywords:
Cleidion brevipetiolatum pax et Hoffm.;
chemical constituents;
isolation and identification;
anti-inflammatory activities
- From:
Chinese Traditional Patent Medicine
2024;46(5):1533-1539
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from Cleidion brevipetiolatum pax et Hoffm.and their anti-inflammatory activities.METHODS The extract from C.brevipetiolatum was isolated and purified by silica gel,MCI,Rp-18,Sephadex LH-20,preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The MTT and Griess methods were used to evaluate the anti-inflammatory activities of compounds.RESULTS Seventeen compounds were isolated and identified as cleomiscosin C(1),scopoletin(2),fraxedin(3),isofraxidin(4),luteolin(5),apigenin(6),chrysoeriol(7),1-hydroxy-2-O-β-D-glucopyranosyl-4-allylbenzene(8),1-O-β-D-glucopyranosyl-4-allylbenzene(9),trans-1-(4-propenyl)-phenol-β-D-glucopyranosyl(10),benzyl-O-β-D-glucopyranoside(11),2-phenylethyl-1-O-β-D-glucopyranoside(12),(+)-syringaresinol(13),aurantiamide(14),(S)-(+)-2-cis-abscisic acid(15),loliolide(16),and hydroxychavicol(17).The ethanol extract of C.brevipetiolatum and its ethyl acetate portion showed NO inhibition with IC50 values of(44.11±5.29),(24.25±3.59)μg/mL,respectively.The IC50 values of compounds 2,and 5-7 were 3.55-14.53 μmol/L.CONCLUSION Compounds 1-7 and 13-17 are isolated from this plant for the first time.Simple coumarins and flavones from this plant show good inhibition of the production of NO.
