Simultaneous content determination of ten constituents in Yuejihua Formula Granules by HPLC-MS/MS
10.3969/j.issn.1001-1528.2024.05.003
- VernacularTitle:HPLC-MS/MS法同时测定月季花配方颗粒中10种成分的含量
- Author:
Zhi-Jiao LI
1
;
Zhen-Qiu ZHENG
;
Xiao-Bo ZHANG
;
Chang-Qin GONG
Author Information
1. 临沂市检验检测中心,山东 临沂 276001
- Keywords:
Yuejihua Formula Granules;
chemical constituents;
content determination;
HPLC-MS/MS
- From:
Chinese Traditional Patent Medicine
2024;46(5):1435-1440
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC-MS/MS method for the simultaneous content determination of hyperoside,isoquercitrin,gallic acid,ellagic acid,quercetin,rutin,afzelin,avicularin,quercetin-3-O-6-trans-chumaryl-β-D-glucoside and quercetin 3-O-2″-galloyl-β-D-glucoside in Yuejihua Formula Granules.METHODS The analysis was performed on a 40℃ thermostatic Water Acquisition UPLC ? HSS T3 column(1.8 μm,2.1 mm×150 mm),with the mobile phase comprising of methanol-0.1%formic acid flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in negative ion scanning with multiple reaction monitoring mode.RESULTS Ten constituents showed good linear relationships within their own ranges(r＞0.995 8),whose average recoveries were 97.3%-99.6%with the RSDs of 0.8%-2.7%.CONCLUSION This simple,rapid,sensitive and specific method can be used for the quality control of Yuejihua Formula Granules.
