Simultaneous content determination of twelve constituents in Guanhuangmu Granules by UPLC-MS/MS
10.3969/j.issn.1001-1528.2024.05.002
- VernacularTitle:UPLC-MS/MS法同时测定关黄母颗粒中12种成分的含量
- Author:
Kuang-Yi LIU
1
;
Ling-Yun ZHANG
;
Xiu-Ting YANG
;
Mi PENG
;
Chuan-Zhi TU
Author Information
1. 南昌市第一医院药剂科,江西 南昌 330000
- Keywords:
Guanhuangmu Granules;
chemical constituents;
content determination;
UPLC-MS/MS
- From:
Chinese Traditional Patent Medicine
2024;46(5):1430-1434
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of hyperoside,kaempferol-3-O-rutinoside,esculin,chlorogenic acid,berberine,quercetin,rehmannioside D,palmatine,leonurin A,paeoniflorin,timosaponin BⅡand rutin in Guanhuangmu Granules.METHODS The analysis was performed on a 40℃ thermostatic Acquity UPLC HSS T3 column(100 mm×2.1 mm,1.8 μm),with the mobile phase comprising of 0.05%formic acid(containing 2 mmol/L ammonium acetate)-acetonitrile flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning.RESULTS Twelve constituents showed good linear relationships within their own ranges(r＞0.999 0),whose average recoveries were 93.46%-102.01%with the RSDs of 2.13%-3.65%.CONCLUSION This rapid,accurate,stable and sensitive method can be used for the quality control of Guanhuangmu Granules.
