Simultaneous content determination of fourteen constituents in Waigan Fengsha Granules by UPLC
10.3969/j.issn.1001-1528.2024.05.001
- VernacularTitle:UPLC法同时测定外感风痧颗粒中14种成分的含量
- Author:
Wen LIU
1
;
Hui-Jie QIN
;
Ting HE
;
Li-Wen LU
;
Yao-Hua LI
Author Information
1. 广西中医药大学药学院,广西 南宁 530001
- Keywords:
Waigan Fengsha Granules;
chemical constituents;
content determination;
UPLC;
chemometrics
- From:
Chinese Traditional Patent Medicine
2024;46(5):1425-1429
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a UPLC method for the simultaneous content determination of chlorogenic acid,protocatechuic acid,protocatechualdehyde,cryptochlorogenic acid,syringic acid,1,3-dicaffeoylquinic acid,cynaroside,isochlorogenic acid B,neochlorogenic acid,isochlorogenic acid A,hesperidin,caffeic acid,isochlorogenic acid C and rosmarinic acid in Waigan Fengsha Granules.METHODS The analysis was performed on a 30℃ thermostatic Waters ACQUITY UPLC.HSS C18 column(2.1 mm×100 mm,1.8 μm),with the mobile phase comprising of acetonitrile-0.2%phosphoric acid flowing at 0.2 mL/min in a gradient elution manner,and the detection wavelengths were set at 275,327 nm.Subsequently,chemometric analysis was performed.RESULTS Fourteen constituents showed good linear relationships within their own ranges(r≥0.999 6),whose average recoveries were 95.68%-104.8%with the RSDs of 0.7%-3.0%.Seven batches of samples were clustered into two types,three principal components demonstrated the acumulative contribution rate of 93.031%.CONCLUSION This accurate and reliable method can be used for the quality control of Waigan Fengsha Granules.
