Effect of different expression levels of GRIM-19 on the resistance of prostate cancer cells to docetaxel chemotherapy
10.13263/j.cnki.nja.2024.10.004
- VernacularTitle:GRIM-19不同表达水平对前列腺癌细胞多西他赛化疗耐药性的影响
- Author:
Hai-Li LIN
1
;
Yong-Xin HE
;
Tian-Qi LIN
;
Zai-Xiong SHEN
;
Liu-Tao LUO
;
Si-Xing HUANG
;
Yi HUANG
;
Yu ZHOU
;
Min-Yi RUAN
Author Information
1. 福建医科大学附属漳州市医院,泌尿外科,福建漳州 363000
- Keywords:
prostate cancer;
GRIM-19;
docetaxel;
drug resistance
- From:
National Journal of Andrology
2024;30(10):884-888
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of GRIM-19 on the resistance of carcinoma cells to the chemotherapeutic agent docetaxel in the treatment of PCa.Methods:Using siRNA technology to interfere with the gene expression in PCa cells,we estab-lished a model of GRIM-19 overexpression/knockdown in PCa cells.We investigated the effect of different expression levels of GRIM-19 on docetaxel-induced death of the PCa cells by qPCR,Western blot and flow cytometry,and assessed the value of GRIM-19 in re-ducing the chemotherapy-resistance of PCa cells.Results:GRIM-19 was down-regulated in PCa tissues and cells.Knockout of GRIM-19 significantly decreased the expression of siGRIM19 in the PC-3 and LNCaP cells,and reduced their death rate when treated with docetaxel compared with the control group.The expressions of GRIM-19 mRNA and protein were remarkably upregulated after transfection with GRIM-19,and the overexpressed GRIM-19 promoted the death of the PC-3 and LNCaP cells treated with docetaxel in a dose-dependent manner.Flow cytometry analysis showed a lower apoptosis rate of PC-3-R cells than that of PC-3 cells at different time points of docetaxel-induction at different doses.Conclusion:GRIM-19 is a PCa suppressor gene with a significant facilitating effect on the apoptosis of PCa cells,and the overexpression of GRIM-19 promotes docetaxel-induced PCa cell death and improves the sensitivity of chemotherapy.
