Gene expression characteristics of lncRNAs and mRNAs in the sperm of asthenospermia patients
10.13263/j.cnki.nja.2024.09.002
- VernacularTitle:弱精子症患者精子lncRNA与mRNA基因表达特征分析
- Author:
Shui-Bo SHI
1
;
Long-Hua LUO
;
Lian LIU
;
Xue-Ming HUANG
;
Su-Ping XIONG
;
Dan-Dan SONG
;
Dong-Shui LI
Author Information
1. 南昌大学第一附属医院男科,江西南昌 330006
- Keywords:
long non-coding RNA;
messenger RNA;
asthenozoospermia;
RNA sequencing;
differential expression
- From:
National Journal of Andrology
2024;30(9):782-788
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the differential expressions of long non-coding RNA(lncRNA)and messenger RNA(mRNA)in normal and asthenospermia(AS)men and analyze their biological significance in AS.Methods:We isolated and ex-tracted total RNAs from 9 normal and 9 AS sperm samples,determined the expressions of RNAs in the sperm using the DNBSEQ se-quencing platform,and analyzed their relevant functions by gene ontology enrichment(GO)and Kyoto encyclopedia of genes and ge-nomes pathway(KEGG)analyses.Results:An average of 10.64G data was generated per group,with 282 185 RNAs detected,in-cluding 107 009 lncRNAs.Among the total number of lncRNAs,15 157 were differentially expressed,2 190 upregulated and 12 967 downregulated;and among the 19 514 mRNAs,13 736 were differentially expressed,4 995 upregulated and 8 741 downregulated.Differentially expressed genes were enriched mainly in the sperm cell membrane and the pathways related to the ion channel functions,sperm development and fertilization.Conclusion:Differentially expressed lncRNAs and mRNAs can be identified by sequencing a-nalysis of AS and normal sperm.Regulation of sperm function through membrane ion channels may contribute to the development of AS,which provides a molecular basis for further research on AS.
