Impact of sperm DNA fragmentation index on embryonic development and live birth outcome of in vitro fertilization-embryo transfer
10.13263/j.cnki.nja.2024.08.008
- VernacularTitle:精子DNA碎片对体外受精-胚胎移植妊娠结局及胚胎发育的影响
- Author:
Xuan HUANG
1
;
Cen-Cen WANG
;
Hui WANG
;
Bing YAO
;
Li CHEN
Author Information
1. 南京大学附属金陵医院/东部战区总医院生殖医学科,江苏南京 210002
- Keywords:
in vitro fertilization-embryo transfer;
sperm DNA fragmentation index;
semen parameters;
embryonic development;
pregnancy outcome
- From:
National Journal of Andrology
2024;30(8):722-729
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of sperm DNA fragmentation index(DFI)on embryonic development and live birth outcomes of in vitro fertilization-fresh embryo transfer(IVF-ET).Methods:We selected 947 cases of IVF-ET due to female factors from June 2015 to December 2020 and,according to the DFI,divided them into a low-(DFI＜15％),a medium-(15≤DFI≤30％)and a high-DFI(30％＜DFI)group.We obtained the female factors(including age,body mass index[BMI],and base-line levels of sexual hormones and anti-Müllerian hormone[AMH]),male factors(including age,BMI,and semen parameters),pa-rameters of superovulation induction,semen parameters before and after IVF treatment,outcome of ovulation induction,embryo quality and pregnancy outcome,and compared them among the three groups.Results:The patients in the medium-and high-DFI groups had a significantly older age than those in the low-DFI group.There were statistically significant differences among the three groups in the baseline percentages of progressively motile sperm,non-progressively motile sperm and immotile sperm,and total sperm motility and progressive sperm motility before IVF(P＜0.01).The baseline percentage of morphologically normal sperm,sperm concentration and total sperm count before IVF treatment,and sperm concentration after IVF treatment were significantly lower in the medium-and high-DFI groups than in the low-DFI group,and so was the total sperm count in the high-DFI than in the low-and medium-DFI groups either before or after semen treatment(P＜0.01).The fertilization rate was also markedly lower in the medium-and high-DFI than in the low-DFI group,but no statistically significant differences were observed in ovulation induction parameters,embryo quality and pregnan-cy outcome among the three groups.Conclusion:High DFI significantly affects sperm motility.The DFI level after semen treatment is negatively correlated with sperm concentration,total sperm count and fertilization rate,but does not affect embryo quality and preg-nancy outcome.
