Differential expressions of seminal plasma piRNAs in men and its significance
10.13263/j.cnki.nja.2023.03.004
- VernacularTitle:精浆piRNAs在人群中的差异表达及其作用机制的初步探索
- Author:
Zhi-Xiang GAO
1
,
2
;
Hua-Pei WANG
;
Neng-Liang DUAN
;
Yuan-Shuai RAN
;
Feng-Mei CUI
;
Qiu CHEN
;
Bo-Xin XUE
;
Xiao-Long LIU
Author Information
1. 苏州大学附属第二医院泌尿外科,江苏苏州 215000
2. 苏州大学附属张家港医院泌尿外科,江苏张家港 215600
- Keywords:
piRNAs;
male infertility;
energy metabolism;
spermatogenesis
- From:
National Journal of Andrology
2023;29(3):218-226
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the differential expressions of piRNAs in the seminal plasma of men and the role of piRNAs in spermatogenesis.Methods:We sequenced the seminal plasma samples collected from 187 male infertility patients and 58 normal healthy men,obtained differentially expressed piRNAs,and detected the relative expressions of piRNAs in different types of sperm by RT-qPCR to explore their significance in the diagnosis of male infertility.Using histopathology,RNA-protein pull-down and Western blot,we investigated the action mechanism of piRNAs in spermatogenesis in the mouse model.Results:RT-qPCR of the seminal plasma samples revealed a high expression of hsa_piR_000478 in teratozoospermia and ROC curve analysis showed an auxiliary signifi-cance of hsa_piR_000478 in the diagnosis of the disease(AUC=0.7549).Transfection of hsa_piR_000478 and its homologous se-quence piR_mmu_54800729 into the seminiferous tubules of the mouse model significantly decreased sperm motility,increased the per-centage of morphologically abnormal sperm and destroyed the testicular structure.Molecular biological experiments exhibited a close correlation between piRNAs and the energy metabolism-related pathway,which elevated the level of cell glycolysis and interfered with normal spermatogenesis.Conclusion:hsa_piR_000478 has an auxiliary significance in the diagnosis of male infertility,and piRNAs may interfere with spermatogenesis by affecting the glycolysis-related pathway in the spermatogenic microenvironment of the testis.
