Lutein inhibits the adhesion,invasiveness and metastasis of human prostate cancer PC-3M cells
10.13263/j.cnki.nja.2023.03.003
- VernacularTitle:叶黄素抑制人前列腺癌PC-3M细胞黏附和转移
- Author:
Guang LIU
1
;
You-Gen HU
;
Yun-Bing WU
;
Wei-Yuan SHAN
;
Yuan-Jie TANG
Author Information
1. 武警江苏总队医院泌尿外科,江苏扬州 225003
- Keywords:
prostatic cancer;
lutein;
cell adhesion;
invasion;
metastasis
- From:
National Journal of Andrology
2023;29(3):210-217
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of lutein on the adhesion,invasiveness and metastasis of human prostate cancer PC-3M cells and its action mechanism.Methods:We divided human prostate cancer PC-3M cells into a control,a low-dose lutein,a medium-dose lutein and a high-dose lutein group,and treated them with 0,10,20 and 40 μmol/L lutein,respectively.Then we ex-amined the adhesion of the cells to matrix by cell adhesion assay and the changes in cell pseudopodia by Phalloidin staining,detected the expressions of paxillin,matrix metalloproteinase 2(MMP-2),MMP-9,recombinant tissue inhibitors of metalloproteinase 1(TIMP-1),E-cadherin,N-cadherin and vimentin by Western blot,determined the invasiveness and migration of the cells by scratch and Tran-swell assays,and observed their dynamic movement by high-intension imaging.Results:Compared with the control,the lutein inter-vention groups showed significant reduction in the number of the cells adhered to matrix,the number of cell pseudopodia,the expres-sions of paxillin,MMP-2,MMP-9,N-cadherin and vimentin,the rates of migration,invasion and metastasis,and the distances of dis-placement and movement of the cells.However,the expressions of TIMP-1 and epithelial-mesenchymal transition-related E-cadherin were upregulated significantly.Conclusion:Lutein can inhibit cell adhesion,reduce the expressions of MMPs,and suppress cell in-vasion and migration by inhibiting the proeess of epithelial-mesenchymal transition.
