Biological mechanisms of Oxalis corniculata regulating human prostate cancer PC-3 cells:An investigation based on the NF-κB pathway
10.13263/j.cnki.nja.2023.03.002
- VernacularTitle:基于NF-κB通路探讨苗药酢浆草调控人前列腺癌PC-3细胞生物学作用的机制研究
- Author:
Guo-Wei ZHANG
1
;
Ming-Wei ZHAN
;
Xu-Xin ZHAN
;
Yi YU
;
Peng-Fei LIU
;
Lei WANG
;
Hao WU
;
Can-Qin HE
;
Qiang LOU
;
Xue-Jun SHANG
Author Information
1. 宿迁市第一人民医院泌尿外科,江苏宿迁 223800
- Keywords:
Oxalis corniculata;
PCa;
PC-3 cell;
NF-κB
- From:
National Journal of Andrology
2023;29(3):202-209
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the biological mechanisms underlying the effect of the Chinese herbal medicine Oxalis cor-niculata on human prostate cancer PC-3 cells.Methods:Through in vitro experiment,we treated human prostate cancer PC-3 cells with different concentrations of Oxalis corniculata,assessed the viability of the cells by MTT assay,examined their apoptosis by flow cytometry,evaluated their migration and invasiveness by Transwell assay,and determined the expressions of the proteins p65,p-p65,IκBα and p-IκBα in the NF-κB pathway using protein imprinting technology.Results:Compared with the blank control,Oxalis cor-niculata significantly inhibited the proliferation and induced the apoptosis of the PC-3 cells(P＜0.05),suppressed their migration and invasiveness in a dose-dependent manner(P＜0.05),and upregulated the expression of IκBα and downregulated those of p-p65 and p-IKBα in the NF-κB pathway(P＜0.05).Conclusion:Oxalis corniculata can inhibit the proliferation,migration and inva-siveness and induce the apoptosis of human prostate cancer PC cells,which may be attributed to its abilities of inhibiting the expres-sions of p-p65 and p-IκBα and regulating the activity of the NF-κB pathway.
