Meiotic Resumption of Isolated Mouse Germinal Vesicle in Cell-freeSystem
10.3969/j.issn.1009-3591.2001.02.002
- VernacularTitle:非细胞体系小鼠卵母细胞游离生发泡减数分裂的启动
- Author:
DAI GU
1
;
LI CHAOJUN
;
BI CHUNMING
;
Chen YAN
;
WU YAOCHUN
;
ZHANG XIRAN
Author Information
1. Life Science College of Nanjing Normal University
- From:
National Journal of Andrology
2001;7(2):79-83
- CountryChina
- Language:Chinese
-
Abstract:
Objectives: To establish an effective method to isolate germinal vesicle frommammalian oocyte and make the isolated germinal vesicle undergo meiotic resumptionin vitro. Methods: The germinal vesicles were isolated from oocytes directly througha physical method by drawing oocyte in and out of fine-bore pipette.The isolatedgerminal vesicles were cultured in cell-free system which was prepared from the celllysate of synchronized HeLa cells.DNA fluorescence dye Hoechest 33342 was used tomonitor the condensation of chromatin during germinal vesicle breakdown. Results:The cell lysate of metaphase HeLa cell induced chromatin condensation while the cellextract of early G2 phase could not. Conclusions: The germinal vesicle can beisolated successfully in such a physical method and it can undergo chromatincondensation in vitro just as the oocyte does in vivo. Natl J Androl,2001,7(2):79～83
