The role and mechanism of vitamin D supplementation in an animal model of Hashimoto′s thyroiditis
10.3760/cma.j.cn311282-20231026-00136
- VernacularTitle:补充维生素D在桥本甲状腺炎动物模型中的作用及其机制研究
- Author:
Fang FANG
1
;
Yaxin LIU
;
Wenli FENG
;
Hongwei JIA
;
Qing HE
;
Mei ZHU
Author Information
1. 天津医科大学总医院内分泌代谢科，国家卫生健康委员会激素与发育重点实验室，天津市代谢性疾病重点实验室，天津 300052
- Keywords:
Hashimoto′s thyroiditis;
Vitamin D;
CD4 + T cells
- From:
Chinese Journal of Endocrinology and Metabolism
2024;40(6):515-520
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and mechanism of vitamin D supplementation on Hashimoto′s thyroiditis(HT).Methods:Female SD rats were randomly divided into four groups by random number table method: control group, experimental autoimmune thyroiditis(EAT) control model group(model group), low-dose(VD1 group) and high-dose(VD2 group) active vitamin D intervention groups. The morphology of thyroid cells, thyroid function, thyroid antibodies, various CD4 + T cells, and related cytokine levels among different groups were compared. Results:The levels of thyroid peroxidase antibody(TPOAb) and thyroid globulin antibody(TgAb) in model group were significantly higher than those in control group, while the levels of VD1 and VD2 groups were significantly lower than those in model group( P<0.05). Compared with control group, HE staining in model group showed severe damage of follicular epithelial cells; Compared with model group, the degree of atrophy and destruction of follicular epithelial cells in VD1 and VD2 groups were reduced. The proportion of helper T cell(Th)1 and Th17 cells and related cytokine levels in model group were significantly higher than those in control group, while those in VD1 and VD2 groups were lower than those in model group( P<0.05); The proportion of regulatory T cell(Treg) cells and related cytokine levels in model group were significantly lower than those in control group, while those in VD1 and VD2 groups were higher than those in model group( P<0.05). Conclusions:After supplementing with vitamin D, the levels of TPOAb and TgAb in EAT rats decreased, and the number of various CD4 + T cells and related cytokine levels tended towards normalization. This suggests that vitamin D may improve HT by regulating CD4 + T cell differentiation, providing a theoretical basis for the role of vitamin D supplementation in HT treatment.
