Changes in FOXO1 expression in β-cells during the progression of type 2 diabetes mellitus and aging
10.3760/cma.j.cn311282-20240210-00063
- VernacularTitle:β细胞中FOXO1表达在2型糖尿病进程和衰老中的改变
- Author:
Rui LIANG
1
;
Tengli LIU
;
Jiaqi ZOU
;
Boya ZHANG
;
Shusen WANG
;
Na LIU
Author Information
1. 天津市第一中心医院卫健委危重病急救医学重点实验室，天津 300384
- Keywords:
Diabetes mellitus;
β-Cell dedifferentiation;
Aging;
HbA 1C
- From:
Chinese Journal of Endocrinology and Metabolism
2024;40(5):414-419
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the changes of β-cell dedifferentiation in type 2 diabetes mellitus(T2DM) and aging subjects.Methods:Using pancreatic samples from 12 prediabetic individuals, 21 type 2 diabetic patients, and 27 control, the level of β-cell dedifferentiation was assessed by immunofluorescence staining for FOXO1 and insulin expression. Correlation analyses were performed between β-cell dedifferentiation levels and hemoglobin A 1C(HbA 1C) level in 60 human pancreatic samples. Correlation analyses were performed between β-cell dedifferentiation levels and age in non-diabetic and T2DM. Results:FOXO1 was mainly expressed in the cytoplasm of human islet β-cells. The proportion of FOXO1 -INS + /INS + cells in T2DM significantly increased compared with control and pre-diabetes, and positively correlated with HbA 1C level( r=0.623, P<0.001). The proportion of FOXO1 -INS + /INS + cells in the young group of T2DM was significantly higher than that in the non-diabetic young and elderly groups, and further significantly increased in elderly group. In T2DM, the proportion of FOXO1 -INS + /INS + cells was positively correlated with age( r=-0.53, P<0.05). Conclusion:Hyperglycemia and aging lead to an increased level of β-cell dedifferentiation in T2DM.
