Effect of right stellate ganglion block on acute lung injury induced by hind limb ischemia-reperfusion in rats
10.3760/cma.j.cn131073.20240229.00609
- VernacularTitle:右侧星状神经节阻滞对后肢缺血再灌注诱发大鼠急性肺损伤的影响
- Author:
Huanlong GAO
1
;
Yadong ZHANG
;
Nan XIN
;
Haitao TIAN
;
Yanli WANG
Author Information
1. 济宁市第一人民医院麻醉科，济宁 272000
- Keywords:
Stellate ganglion;
Nerve bock;
Reperfusion injury;
Acute lung injury
- From:
Chinese Journal of Anesthesiology
2024;44(6):694-697
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of the right stellate ganglion block (SGB) on the acute lung injury induced by hind limb ischemia-reperfusion (I/R) in rats.Methods:Twenty-seven SPF healthy male Sprague-Dawley rats, aged 7-8 weeks, weighing 200-250 g, were divided into 3 groups ( n=9 each) by the random number table method: sham operation group (Sham group), hind limb I/R group (I/R group) and right SGB+ hind limb I/R group (SGB+ I/R group). The right hind limb I/R injury model was developed in anesthetized rats in I/R and SGB+ I/R groups, and the right SGB was performed with 0.2% ropivacaine 0.5 ml before the right hind limb ischemia in SGB+ I/R group. The pathological changes of lung tissues were observed with a light microscope, the wet/dry lung weight ratio, PaO 2 and PaCO 2 were determined, and the oxygenation index was calculated. The concentrations of serum malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were determined using enzyme-linked immunosorbent assay. Results:Compared with Sham group, the wet/dry lung weight ratio, PaO 2, oxygenation index, and GSH-Px concentrations were significantly decreased, and the concentrations of serum MDA were increased in I/R and SGB+ I/R groups ( P<0.05). Compared with I/R group, the wet/dry lung weight ratio, PaO 2, oxygenation index, and GSH-Px concentrations were significantly increased, the concentrations of serum MDA were decreased ( P<0.05), and the pathological changes of lung tissues were significantly attenuated in SGB+ I/R group. There was no significant difference in PaCO 2 among the 3 groups ( P>0.05). Conclusions:The right SGB can alleviate the acute lung injury induced by hind limb I/R, and the mechanism may be related to the inhibition of oxidative stress responses in rats.
