Aspirin alleviates OGD/R-induced injury in mouse hippocampal neurons by regulating ferroptosis
10.3969/j.issn.1009-0126.2024.08.022
- VernacularTitle:阿司匹林通过铁死亡减轻氧糖剥夺复氧的小鼠海马神经元细胞损伤的作用机制
- Author:
Yujiao HU
1
;
Shan CONG
;
Lei ZHAO
;
Chunxue DONG
;
Dongmei WANG
;
Nannan WANG
;
Ying MAO
Author Information
1. 161006 齐齐哈尔医学院附属第二医院神经内二科
- Keywords:
brain ischemia;
reperfusion injury;
aspirin;
ferroptosis;
hippocampus;
neurons;
oxygen glucose deprivation/reoxygenation
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2024;26(8):960-964
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of aspirin on oxygen glucose deprivation/reoxygen-ation(OGD/R)-induced injury in mouse hippocampal neuron HT22 cells by regulating ferropto-sis.Methods HT22 cells were randomly divided into control group,model group,low-,medium-and high-dose groups(n=3).Cellular OGD/R injury model was established in the other 4 groups except the control group.Aspirin of 100,200 and 400 μg/ml was used to treat the cells from the above 3 treatment groups,respectively.Cell viability was detected by CCK-8 assay.The contents of TNF-α,IL-1β and IL-6 were detected by ELISA.The contents of SOD,catalase,glutathione,reac-tive oxygen species(ROS),lactate dehydrogenase(LDH),Fe2+and MDA were detected by the corresponding reagent kits.Western blot analysis was performed to determine the expression of solute carrier family 7 members 11(SLC7A11),glutathione peroxidase 4(GPX4)and Acyl-CoA synthase long chain member 4(ACSL4).Results The model group had significantly lower cell vi-ability than the control group(0.49±0.07 vs 1.00±0.12,P＜0.01),but the viability of the low-,medium-and high-dose groups were higher than that of the model group(0.72±0.10 vs 0.49±0.07,P＜0.05;0.87±0.10 vs 0.49±0.07,P＜0.01;0.93±0.07 vs 0.49±0.07,P＜0.01).Compared with the control group,the contents of TNF-α,IL-1β,IL-6,ROS,LDH,Fe2 and MDA and the protein expression of ACSL4 were significantly increased,while the contents of SOD,catalase,glutathione and protein levels of SLC7A11 and GPX4 were obviously decreased in the model group(P＜0.01).Compared with model group,aspirin treatment reversed all above indicators no matter its doses(P＜0.05,P＜0.01).Conclusion Aspirin alleviates OGD/R-induced neuronal in-jury through regulating ferroptosis in mouse neuron HT22 cells in a dose-dependent manner.
