Apoptotic Effects of 6-Gingerol in LNCaP Human Prostate Cancer Cells.
- Author:
Hyun Woo KIM
1
;
Deuk Hee OH
;
Chaeyong JUNG
;
Dong Deuk KWON
;
Young Chai LIM
Author Information
1. Department of Pharmacology, Chonnam National University Medical School, Gwangju, Korea. limyc@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
6-Gingerol;
Apoptosis;
Caspase-3;
Poly(ADP-ribose) Polymerases;
Human prostate cancer cells
- MeSH:
Apoptosis;
Blotting, Western;
Caspase 3;
Catechols;
Cell Cycle Checkpoints;
Cell Line;
Cell Survival;
Fatty Alcohols;
Flow Cytometry;
Ginger;
Humans;
Poly(ADP-ribose) Polymerases;
Prostate;
Prostatic Neoplasms
- From:Soonchunhyang Medical Science
2011;17(2):75-79
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: 6-Gingerol, one component of ginger (Zingiber officinale) compound, has been known to possess anti-inflammatory, analgesic, anti-emetic, and anti-cancer effects. In this study, the apoptotic ability of 6-gingerol was investigated in human prostate cancer cells. METHODS: 3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide (MTT) assay, flow cytometry, and western blot analysis were done in LNCaP human prostate cancer cell lines treated with the various doses of 6-gingerol for the different durations of drug exposure. RESULTS: 6-Gingerol in doses ranging from 100 to 300 microM induced dose- and time-dependent inhibition of cell viability in prostate cancer cells by using MTT assay. Maximal inhibition of cell viability was observed at 300 microM of 6-gingerol for 48 hours treatment in LNCaP cells. 6-Gingerol at the dose of 100 microM did not produce any significant change in apoptotic cells in flow cytometry analysis. However, significant increase in sub-G0/G1 phase was observed in cells treated with 200 and 300 microM of 6-gingerol. Any significant cell cycle arrest was not induced by 6-gingerol. In western blotting analysis, expression of caspase-3 was not evident in cells treated with 6-gingerol for 24 hours. However, 48 hours treatment with 6-gingerol altered the expression of caspase-3 in LNCaP cells. Expression of cleaved poly showed the dose-dependent fashion in both 24 hours and 48 hours treatment of 6-gingerol. CONCLUSION: These observations suggest that 6-gingerol may induce apoptosis in LNCaP human prostate cancer cells.
