Comparative Analysis of Multiplex PCR and Hybrid Capture System in the Detecttion of Human Papillomavirus in the Uterine Cervix.
- Author:
Sei Jun HAN
1
;
San KIM
;
Min Chang KANG
;
Ju Seong LIM
;
Tae Gyu AHN
;
Byung Rai LEE
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Chosun University, Gwangju, Korea.
- Publication Type:Original Article
- Keywords:
HPV;
Multiplex PCR;
Hybrid capture I
- MeSH:
Cervix Uteri*;
Female;
Humans*;
Multiplex Polymerase Chain Reaction*;
Prevalence;
Uterine Cervical Neoplasms
- From:Korean Journal of Obstetrics and Gynecology
2003;46(1):28-33
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To compare the multiplex-PCR and hybrid capture methods for detection of Human papillomavirus (HPV) in uterine cervical swab samples. METHODS: This study determined the HPV infection and its risk group (subtype) in uterine cervical swab samples of 91 Korean women by HPV hybrid capture I and multiplex-PCR method. RESULTS: The prevalence of HPV infection determined by muliplex-PCR was more higher than by hybrid capture method I. Of 65 women with nonspecific uterine cervical lesions, the frequence of HPV-positivity was 14/65 (12 low-risk and 2 high-risk HPV) by hybrid capture method I and was 37/65 (34 low-risk and 3 high- risk HPV) by multiplex-PCR method, respectively. The frequence of high-risk HPV was 2/6 in LSIL, 7/14 in HSIL, 4/6 in uterine cervical cancer by hybrid capture method I, and was 2/6 in LSIL, 8/14 in HSIL, 4/6 in uterine cervical cancer by multiplex-PCR method. CONCLUSION: These results suggest that multiplex-PCR is more sensitive, simple and cost-effective technique than Hybrid-capture I in the detection of HPV infection in the uterine cervix.
