Telomerase regulation factor PinX1 inhibits proliferation and invasion of hepatoma cells
10.3877/cma.j.issn.2095-3232.2018.02.015
- VernacularTitle:端粒酶调控因子PinX1抑制肝癌细胞增殖与侵袭
- Author:
Ruixi LI
1
;
Zhicheng YAO
;
Zhiyong XIONG
;
Boxuan ZHOU
;
Jianliang XU
;
Kunpeng HU
;
Weiming FAN
;
Hao LIANG
;
Meihai DENG
Author Information
1. 中山大学附属第三医院肝胆外科
- Keywords:
Carcinoma,hepatocellular;
Telomerase;
PinX1;
hTERT
- From:
Chinese Journal of Hepatic Surgery（Electronic Edition）
2018;7(2):147-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the impact of telomerase regulation factor PinX1 to the proliferation and invasion ability of hepatoma cells. Methods Hepatoma cells PinX1-7721 (experimental group) with stable expression of PinX1 as well as control cell VECTOR-7721 (control group) were constructed. The expression of PinX1 mRNA was detected by RT-PCR. The proliferation ability and clonality of hepatoma cells were detected by CCK-8 method and plate clonality assay, and the invasion ability of hepatoma cells by Transwell assay. Comparison of the experiment data was conducted by t test. Results Expression level of PinX1 mRNA in experiment group was (13.9±2.0)×10-3, which was significantly higher than (1.1±0.2)×10-3in control group (t=10.98, P<0.05). A450of the cells on 1-7 d in experiment group was respectively 0.260±0.004, 0.340±0.008, 0.450±0.040, 0.500±0.020, 0.730±0.030, 1.350±0.040 and 1.640±0.050, which were significantly lower than 0.280±0.009, 0.410±0.007, 0.680±0.044, 0.730±0.029, 0.850±0.070, 1.700±0.020 and 2.080±0.280 in control group (t=-5.82, -12.99, -6.36, -5.96, -28.42,-18.98, -5.08; P<0.05). The plate clonality assay results showed that the clone formation quantity of cells in experiment group was 143±32, which was significantly lower than 305±25 in control group (t=-6.91, P<0.05).Transwell assay results showed that the quantity of trans-membrane cell in experiment group was 230±16, which was significantly lower than 650±30 in control group (t=-21.40, P<0.05). Conclusion PinX1 could inhibit the proliferation and invasion ability of hepatoma cells.
